HiTrap Phenyl FF (high sub) are prepacked Phenyl Sepharose 6 Fast Flow columns for hydrophobic interaction chromatography (HIC). The columns are used for small-scale protein purification as well as for screening of binding and elutions conditions.

• Provide convenient and fast small-scale protein purifications.
• Phenyl Sepharose 6 Fast Flow is a well-established, standard aromatic HIC resin for capture and intermediate purification requiring low to resin hydrophobicity. Two levels of substitution on Phenyl Sepahrose 6 Fast Flow help find optimal selectivity and binding capacity.
• Flexible design for use with syringes, single laboratory pump, or chromatography system such as ÄKTA design.
• High flow rates and high capacities.
• Excellent and easy scale-up achieved by connecting several HiTrap columns in series or using HiPrep columns.