This resin is designed for the binding and elution of relatively strong hydrophobic molecules at comparatively low salt concentrations. Butyl‑S Sepharose 6 Fast Flow is particularly useful during the initial stages of a separation process to remove the bulk of impurities without stringent requirements for conditioning of the sample (e.g. adjustment of pH, salt concentration etc.)

About PreDictor Butyl-S Sepharose 6 Fast Flow plates

PreDictor plates are disposable, 96-well filter plates for parallel screening of resins and chromatographic conditions such as binding, wash, and elution conditions.

• Screen different chromatography resins simultaneously using dedicated screening plates, or more thoroughly using plates containing a single resin.
• Different applications require different amounts of resins in the wells, and we offer a wide range of PreDictor plates containing different chromatography resins at different volumes.
• Data generated using PreDictor plates show good correlation with data from chromatography columns, making the plates an excellent tool for initial screening of process conditions.

Assist software supports the PreDictor workflow from set-up of experimental design to data evaluation.

Prepacked columns and other formats

Butyl-S Sepharose 6 Fast Flow resin is available in the following formats: 

Bulk resin

Loose Butyl-S Sepharose 6 Fast Flow resin in bottles for batch purification or packing your own columns

Process development scale – prepacked

• PreDictor RoboColumn™ Butyl-S Sepharose 6 FF units – miniaturized columns for high-throughput process development.
• HiScreen Butyl-S FF columns – optimized for method and process development.

Lab/research scale – prepacked for manual purification

HiTrap Butyl-S FF columns – for easy use with a syringe, peristaltic pump, or chromatography system.

Lab/research scale – prepacked for automated purification and analysis

HiTrap Butyl-S FF columns – for easy use with a syringe, peristaltic pump, or chromatography system.