A Size Exclusion Column for Efficient, High Resolution Protein Separation

Size exclusion chromatography (SEC) column technologies have played a critical role in biomolecule separation for decades. As protein purification is a common method in labs worldwide, scientists increasingly need reliable and convenient options that meet modern requirements for efficient, high resolution protein separation. HiLoad™ Superdex™ pg SEC columns offer the combination of prepacked convenience and reproducibility together with fast, high-resolution gel filtration at preparative laboratory scale.

Robust resins in a Ready-To-Use Gel Filtration Column

Superdex™ prep grade delivers: • High mechanical strength • High hydrophilicity • High chemical stability • Minimal nonspecific interactions Superdex™ gel filtration resins are produced by the covalent bonding of dextran to highly cross-linked agarose.

The steep selectivity of the dextran component and the high chemical and physical stability of the agarose give high-resolution separations at flow velocities up to 50 cm/h. Scientists also gain excellent resolving power for peptides and proteins in the molecular weight ranges, Mr < 10 000 (Superdex™ 30 pg), Mr 3 000 to 70 000 (Superdex™ 75 pg), and Mr 10 000 to 600 000 (Superdex™ 200 pg). HiLoad™ columns are easy-to-use laboratory columns (Cytiva XK chromatography columns). Each has a precision bore borosilicate glass tube and a fitted thermostatic jacket. Dead volumes make up less than 0.1% of the total column volume, keeping sample dilution and band broadening to a minimum. Valco fittings (1/16”) are standard and provide easy and direct connection to ÄKTA™ protein purification systems. Download the Superdex™ prep grade datafile to learn more.