Protein A resin characteristics

The recombinant protein is produced in E. coli and engineered with an oriented coupling to enhance binding capacity. Epoxy-based coupling ensures low ligand leakage. Specificity of the recombinant protein for the Fc region of IgG is similar to the native protein, giving a high degree of selectivity and yield.

Streamlined scale up

Scale-up with this resin is straightforward and is further aided by a range of prepacked process development tools, making purification scale-up straightforward.

Successful use in published research

Cytiva's protein A resins are used in a variety of applications, including:

• Molecular characterization of an arthritogenic collagen peptide interacting with I-Ar

Compatible empty columns – research scale

Cytiva's solutions include a variety of empty columns for packing. rProtein A Sepharose resin is compatible for use with HiScale, Tricorn, and XK columns.

Compatible empty columns – bioprocess scale

For process-scale applications, Cytiva recommends AxiChrom columns. rProtein A Sepharose Fast Flow can also be packed in the other column platforms such as BPG and Chromaflow.

Convenient prepacked columns – research scale

rProtein A Sepharose Fast Flow is also available in convenient prepacked formats:

• rProtein A GraviTrap, for use with gravity flow
• HiTrap rProtein A FF, for use with a syringe, pump, or chromatography system

Convenient prepacked columns – bioprocess scale

Prepacked ReadyToProcess columns are available on request, eliminating the need for column packing and cleaning validation.