Chemically stable heparin purification resin

Heparin Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads, modified with sodium heparin. Immobilization is performed using chemically stable and oriented coupling, which delivers high binding capacity. The chemical stability of the heparin resin is solely dictated by the ligand.

Streamlined Heparin Sepharose Fast Flow scale-up

Scale-up with this resin is straightforward and is further aided by a range of prepacked process development tools.

Successful use in published research

This heparin resin is used in a variety of research applications, including:

• Study of the biological activity of recombinant human lactoferrin in glycoengineered Pichia pastoris
• Evaluation of the function of the MPH1 gene of saccharomyces cerevisiae in Okazaki fragment processing

Compatible empty columns – research scale

Cytiva's solutions include a variety of empty columns. Heparin Sepharose 6 Fast Flow is compatible with gravity columns, Tricorn columns, XK columns and HiScale columns.

Compatible empty columns – bioprocess scale

For process-scale applications, Cytiva recommends AxiChrom columns. Heparin Sepharose Fast Flow can also be packed in the other column platforms such as BPG and Chromaflow.

Prepacked Heparin Sepharose Fast Flow columns – research scale

Heparin Sepharose Fast Flow resin is available in the following prepacked formats:

• HiTrap Heparin Sepharose 6 FF: easy to use with a syringe, a peristaltic pump, or a chromatography system
• HiPrep Heparin FF: for convenient scale-up

Prepacked Heparin Sepharose Fast Flow columns – bioprocess scale

Prepacked ReadyToProcess columns are available on request. They eliminate the need for column packing and cleaning validation.

Other Cytiva products for affinity purification of proteins

Looking for a different purification solution? Browse Cytiva's resins and prepacked columns for affinity chromatography or let us help you find the right affinity product.