Rolling circle amplification and PCR kits

Hot Start Master Mix is a 2X premixed formulation that can effectively reduce nonspecific priming and primer-dimer formation during PCR.

Preformulated and predispensed, freeze-dried PCR reagents mix including high-quality PuReTaq DNA Polymerase and a hot start activator protein, for increased specificity and reproducibility of PCR amplifications.

RAPD analysis is a technique for rapidly detecting genomic polymorphisms. A single short oligonucleotide primer of arbitrary sequence is used under low stringency conditions in PCR to generate a reproducible array of strain-specific products.

Pre-dispensed, freeze-dried beads that include the reagents necessary for one-step reverse transcription-PCR with high sensitivity and reproducibility.

First-Strand cDNA Synthesis kits are designed to generate full-length first-strand cDNA from mRNA templates following upstream mRNA extraction, isolation, or purification.

GenomiPhi HY DNA Amplification Kit offers highly efficient and representative whole-genome amplification with 40 to 50 μg yield from nanogram amounts of DNA sample.

GenomiPhi HY Ready-To-Go DNA Amplification Kit offers highly efficient and representative whole-genome amplification with 40 to 60 μg yield from nanogram amounts of DNA sample.

Single Cell GenomiPhi DNA Amplification kit combines the unique capabilities of Phi29 DNA polymerase with an optimized formulation to amplify genomic DNA from 1-1000 cells without background interference.

GenomiPhi V2 DNA Amplification Kit offers highly efficient and representative whole-genome amplification with 4 to 7 μg yield from nanogram amounts of DNA sample.

GenomiPhi V3 Ready-To-Go DNA Amplification Kit offers highly efficient and representative whole-genome amplification with 12 to 20 μg yield from nanogram amounts of DNA sample.

PuReTaq RTG PCR beads and reagents ensures reliable and robust performance in both end point and RT-PCR amplifications and ensures the lowest possible levels of contaminating prokaryotic and eukaryotic nucleic acids.

The kit provides 50 preformulated, single-dose reaction beads prepackaged in thin-walled 0.5 ml tubes compatible with most thermal cyclers. For synthesis of first-strand cDNA templates from total RNA or polyadenylated RNA using a primer of choice.

TempliPhi™, based on Phi29 DNA polymerase, is used to exponentially amplify single- or double-stranded circular DNA by rolling circle amplification (RCA).

TempliPhi 2000 Reaction Kit

TempliPhi Large Construct Kit uses isothermal rolling circle amplification (RCA) to efficiently prepare DNA sequencing templates specifically from large DNA vectors (e.g., fosmids and BACs).

TempliPhi™ Sequence Resolver Kit resolves most of the sequencing stops or gaps that routinely elude other commonly used finishing methods. The use of this kit eliminates the need to use dGTP chemistry, which can affect the accuracy of a sequence due to the appearance of sequence compression. In addition, it eliminates the need for additives in the sequencing reaction.