Surface plasmon resonance (SPR) is a biophysical technique for measuring molecular interactions. This method allows the detection of binding interactions with high sensitivity in real time, without the need for labeling. Researchers and biomanufacturers often use SPR to investigate binding kinetics and affinity between molecules.
How SPR works
SPR occurs when polarized light strikes an electrically conducting surface at the interface between two media. This generates electron charge density waves called plasmons, reducing the intensity of reflected light at a specific angle known as the resonance angle, in proportion to the mass on a sensor surface.
During SPR experiments, one molecule, known as the ligand, gets immobilized to a sensor chip made of a glass and a thin layer of a metal, such as gold. Researchers then infuse a solution containing the analyte over the sensor surface. This analyte interacts with the ligand, increasing mass and revealing association kinetics. During the interaction, polarized light is directed toward the sensor surface and the angle of minimum intensity reflected light is detected. This angle changes as molecules bind and dissociate, and the interaction profile is thus recorded in real time in a sensorgram.
Applications of SPR data
The data from an interaction is presented in a sensorgram, providing quantitative data on:
Does the interacting partner bind to the target molecule?
To what extent does an interacting partner cross-react with other molecules?
How much of a given molecule is present and active?
What are the rates of association and dissociation?
How strong is the binding?
This versatile technology supports use as a screening platform and a method for detailed kinetic characterization of interactions. Because of its versatility, SPR is widely used in drug discovery.
SPR instrument software optimizes workflows and processing and reporting of SPR data. Our Biacore™ SPR software range includes:
We also offer extensions and upgrade kits that enhance performance.
Biacore™ Insight Evaluation Software
Biacore™ Insight Evaluation Software is a scalable unified evaluation platform that optimizes the analysis of your SPR data in your Biacore™ system. It is easy to use and supports integration into GxP-regulated workflows. Biacore™ software enables you to visualize and evaluate your kinetic or affinity data. Through supervised machine learning, this software eliminates analysis bottlenecks.
SPR software FAQs
Here are some answers to frequently asked questions about SPR software:
What is SPR software?
SPR software enables the use of SPR instruments as well as data collection and analysis. Different extensions are available for different applications.
What is SPR data?
SPR data refers to the molecular interaction data obtained during surface plasmon resonance experiments.