HiTrap column, 1 mL, with MabSelect VL protein L affinity resin (520x300)

Evaluate binding sites


Affinity chromatography separates proteins based on a reversible binding interaction between the target protein and a ligand. Whether you’re purifying monoclonal antibodies or fragments such as Fabs or scFvs, knowing the binding site and structure of your target molecule is key to a successful purification. Click the figure to learn about protein A, protein L, and other affinity ligands.

Capto™ L resin and MabSelect™ VL has a strong affinity to the variable region of antibody kappa light chains.
MabSelect PrismA™ resin and Fibro PrismA fiber units bind to both the VH and Fc regions.
MabSelect SuRe™ and MabSelect SuRe™ LX resins bind to the Fc region.
MabSelect PrismA™ resin and Fibro PrismA fiber units bind to both the VH and Fc regions.
KappaSelect resin is designed for purification of human Fab (kappa) fragments. LambdaFabSelect resin binds to the constant region of the lambda light chain.
Bispecific antibody image, asymmetric
Antibody affinity resins selection three
Relative remaining DBC using 1.0 M NaOH as cleaning agent at 15 min contact time between cycles.
mAb purification CiPP
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