Evaluate binding sites


Affinity chromatography separates proteins on the basis of a reversible interaction between the target protein and a specific ligand attached to a chromatography base matrix. Knowing where the ligand binds and the structure of your target molecule helps you choose an affinity chromatography capture resin. Click on the figure to learn about different ligands.

Capto™ L resin has a strong affinity to the variable region of antibody kappa light chains.
MabSelect PrismA™ resin and Fibro PrismA fiber units bind to both the VH and Fc regions.
MabSelect SuRe™ and MabSelect SuRe™ LX resins bind to the Fc region.
MabSelect PrismA™ resin and Fibro PrismA fiber units bind to both the VH and Fc regions.
KappaSelect resin is designed for purification of human Fab (kappa) fragments. LambdaFabSelect resin binds to the constant region of the lambda light chain.
Antibody affinity resins selection three
A typical 3-step chromatography scheme for mAb purification
Relative remaining DBC using 1.0 M NaOH as cleaning agent at 15 min contact time between cycles.
Bispecific antibody image, asymmetric
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