FAQ

Spare parts

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# Product Name Product Code Price
1 Net for Columns, i.d 26 mm, 10 µm 18876001 65.14 USD Add to cart
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1 Net for Columns, i.d 16 mm, 10 µm 18876101 65.14 USD Add to cart
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2 Support Screen for Columns, i.d. 26 mm 18937701 65.14 USD Add to cart
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2 Support Screen for Column, i.d. 16 mm 19065101 44.51 USD Add to cart
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3 O-Ring, 10.3 × 2.4 mm 19016301 51.03 USD Add to cart
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3 O-Ring 20.3x 2.62 mm 28978227 70.57 USD Add to cart
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11 Fingertight connector 1/16" male, narrow 28401081 92.28 USD Add to cart
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12 Stop Plug Female, 1/16" 11000464 87.94 USD Add to cart
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Accessories

Make sure you have all connectors and tubing needed for running the column.
Depending on your system configuration and column length you may need extra long tubing to connect your column.
Please do not extend the tubing unnecessarily which results in a lower resolution.

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# Product Name Product Code Price
1 ETFE Tubing, 1.5 m, i.d. 0.75 mm, o.d. 1/16" 18111974 74.73 USD Add to cart
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2 Fingertight connector 1/16" male, narrow 28401081 92.28 USD Add to cart
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3 Union 1/16" female-1/16" female 11000339 137.94 USD Add to cart
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4 Stop Plug Female, 1/16" 11000464 87.94 USD Add to cart
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Troubleshooting

Find solutions to product related issues. For unlisted issues please contact local Cytiva service representation.

Poor reproducibility

Poor product recovery

Columns Column leakage

Back pressure increase during operation

Unusual column appearance

General advice to achieve good performance

Before using the column make sure that:

  • Correct system has been selected in UNICORN System Control
  • Correct wavelength has been set for UV/UPC monitor
  • All tubing has been properly connected and tubing is not longer than needed
  • All connectors are free from leakage, verified by passing a leakage test
  • No tubing is folded or twisted
  • Online filter, if used, is changed on a regular basis
  • Correct buffers are used for the chosen columns and proteins
  • All inlet tubing has been immersed in correct buffer solutions
  • Enough buffer has been prepared
  • Buffers have been equilibrated to the environment temperature
  • Buffers/eluents have been degassed if necessary (e.g., in RPC runs)
  • Suitable columns have been selected for the target proteins
  • Column meets pressure requirements for selected medium
  • Columns have been cleaned and prepared according to column instructions
  • Samples have been clarified by centrifugation and/or filtration prior to sample loading
  • Samples have been adjusted to binding buffer conditions
  • Auto sampler (if used) has been prepared according to user manual
  • The fraction collector has been filled with appropriate number of microtiter plates or tubes
  • Appropriate arrangement for waste handling has been prepared

 

 

Unsatisfactory elution

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