Advanced lab-scale protein purification using CiPP
Course
About course
In this 3-day hands-on course, you will learn advanced applications of ÄKTA™ chromatography systems and UNICORN™ 7.x control software for optimizing lab-scale protein purification. The course will provide an understanding of the relevant chromatography techniques such as affinity, ion exchange, and size-exclusion chromatography.
In the lab, you will use the capture, intermediate, and polishing purification (CiPP) strategy as a basis for planning, executing, and optimizing a multistep purification process of an affinity-tagged recombinant protein (sfGFP) expressed in Escherichia coli.
The course will also give an overview of mechanistic modelling and its application in the optimization of protein purification.
Prior to attending
The course necessitates a basic understanding of handling ÄKTA chromatography systems and UNICORN 7.x control software.
In addition, a basic understanding of computer handling and fast protein liquid chromatography (FPLC) including common techniques like affinity (AC), ion exchange (IEX), or size-exclusion (SEC) chromatography is expected to fulfil the demands of this advanced hands-on training course.
Prior to attending this course, refresh your knowledge on chromatography principles with these free online courses:
- Principles of chromatography
- Get started: protein purification
- Get to know chromatography techniques
- Get started: UNICORN™ software for chromatography
Course dates and location:
Munich, Germany
03-05 March, 2026
16-18 June, 2026
08-10 September, 2026
24-26 November, 2026
After the course, you will be able to:
- Set up ÄKTA chromatography systems and program UNICORN control software for automated two-step purification including scouting, peak-to-loop, and method queues.
- Understand, apply, and optimize common chromatography techniques like affinity (AC), ion exchange (IEX), or size-exclusion (SEC) chromatography.
- Use results to evaluate, interpret, and optimize process parameters accordingly.
Who should attend?
Researchers and technical staff in academia or R&D departments seeking advanced knowledge on using ÄKTA go™, ÄKTA pure™, and ÄKTA avant™ chromatography systems in combination with UNICORN 7.x control software for protein research.
