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Get confident: tagged protein purification

Course

About course

This free online course covers the steps cell culture, sample preparation and tagged protein purification in protein research. 

 

Key learnings: 

- Guidance on how to prepare for purification of proteins tagged with his, GST, Strep tag II and MBP affinity tags 

- Key considerations for selecting a suitable expression system for your application 

- Principle factors of construct design 

- Suitable sample preparation methods for different expression hosts 

 

You´ll also get some troubleshooting tips and learn how to tackle challenges with low expression, inclusion bodies, and protein aggregation. 

 

You can access and complete each lesson individually, with the full course taking around 1.5 hours to complete. 

Certification

You will get an online certificate after the course completion. You can view and download it in a section My Courses. With Cytiva Certificates, portions of our programs have been split into online modules, so you can earn a high quality knowledge in interactive format.

Lessons

Module 1: Introduction to tagged proteins
Interactive content

Locked 13 min
Module 2: General tips
Interactive content

Locked 25 min
Module 3: His-tagged protein purification
Interactive content

Locked 20 min
Module 4: GST-tagged protein purification
Interactive content

Locked 10 min
Module 5: Strep-tag® II-tagged protein purification
Interactive content

Locked 9 min
Module 6: MBP-tagged protein purification
Interactive content

Locked 9 min
Module 7: Purification of other tagged proteins
Interactive content

Locked 5 min
Module 8: Check your knowledge
Interactive content

Locked 5 min
Module 9: Survey - we value your input! 
Interactive content

Locked 0 min
97 min
Certificate available

Author:
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CYT Admin