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Get confident: his-tagged protein purification

Course

About course

In this free interactive course, you’ll learn about the histidine-tag and how it’s used in protein purification. You’ll get practical guidance to help you obtain great results.  

 

Key learnings: 

- How to prepare for purification of his-tagged proteins  

- How to refold proteins from inclusion bodies 

 

This course also includes troubleshooting and tips on how to overcome common chromatography run issues.   

 

Before taking the course, we recommend that you are familiar with the various chromatography techniques used for protein purification. If you need a refresher, check out the online course Get to know: chromatography techniques.  

 

You can access and complete each lesson individually, with the full course taking around 35 minutes to complete. 

 

For more extensive learning on tagged proteins, check out the online course Get confident: tagged protein purification.


Certification

You will get an online certificate after the course completion. You can view and download it in a section My Courses. With Cytiva Certificates, portions of our programs have been split into online modules, so you can earn a high quality knowledge in interactive format.

Lessons

Module 1: Introduction to the his-tag
Interactive content

Locked 1 min
Module 2: Experimental setup
Interactive content

Locked 10 min
Module 3: Tips to get you started
Interactive content

Locked 1 min
Module 4: Refolding of proteins from inclusion bodies
Interactive content

Locked 6 min
Module 5: Application examples
Interactive content

Locked 4 min
Module 6: Troubleshooting
Interactive content

Locked 9 min
Module 7: Summing up
Interactive content

Locked 3 min
Module 8: Check your knowledge
Interactive content

Locked 3 min
Module 9: Survey - we value your input! 
Interactive content

Locked 0 min
38 min
Certificate available

Author:
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CYT Admin