On-line buffer preparation is a great asset when several pH steps must be tested for optimal purification performance or when the amount of protein required from a protocol increases from milligram to gram quantities. The BufferPrep Kit has four extra inlets that help eliminate time-consuming manual buffer preparation and titration usually needed for every pH change in chromatography. Changing buffers manually and moving inlet tubing by hand is no longer necessary. The risk of introducing air into the system is removed. Buffers for cleaning can be added and stored in place for different runs.