HiPrep™ Sephacryl™ S-100 HR column for gel filtration

Prepacked columns for high-resolution protein separation

HiPrep Sephacryl HR columns 16/60 and 26/60

HiPrep 16/60 and 26/60 Sephacryl columns

Sephacryl Lab Packs and HiPrep Sephacryl HR columns 16/60 and 26/60

HiPrep 16/60 and HiPrep 26/60 Sephacryl S100,S-200 and S-300 HR

HiPrep 16/60 and HiPrep 26/60 Sephacryl S-100 HR

Sephacryl media and HiPrep 16/60 and HiPrep 26/60 Sephacryl S-100

Principles and methods: Size Exclusion Chromatography - Fig. 4.21

HiPrep™ Sephacryl™ S-100 HR column for gel filtration

Prepacked columns for high-resolution protein separation

Column i.d.

16 mm

26 mm

HiPrep™ Sephacryl™ S-100 HR column for gel filtration

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Overview

HiPrep Sephacryl S-100 HR are prepacked gel filtration columns for preparative separation of biomolecules from up to 13 mL sample volume. Sephacryl S-100 is used for separating peptides and small proteins.

Maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability resulting from the hydrophilic, rigid allyl dextran/bisacrylamide matrix.
Gel filtration columns for low to medium pressure ÄKTA systems.
Well-established in industrial production.
The HiPrep Sephacryl series offers reproducible separations over a wide molecular weight range.
Convenient, prepacked HiPrep Sephacryl columns speed up protein purification.

Product specifications

Showing 2 products

17116501

In stock6 available

991.00 USD

17119401

In stock10 available

1,331.00 USD

Product availability is not guaranteed and subject to change.

Product Support

FAQ

What is HiPrep Sephacryl S-100 HR used for?

HiPrep™ Sephacryl™ S-100 prepacked chromatography column is used for separating peptides and small proteins.

How does size exclusion chromatography work in HiPrep Sephacryl columns?

Size exclusion chromatography separates molecules based on size by filtration through a gel. The matrix of HiPrep™ Sephacryl™ protein separation columns consists of cross-linked copolymer allyl dextran and N,N'-methylene bisacrylamide. Small molecules are retained in the matrix and large molecules are eluted.

What is the separation range of Sephacryl S-100 HR?

HiPrep™ Sephacryl™ S-100 HR column is designed for separating peptides and small proteins with a fractionation range globular proteins 1 × 10– 1 × 10⁵.

How to use HiPrep Sephacryl columns for protein purification?

HiPrep™ Sephacryl™ columns arrive prepacked and ready to use. They are simple to run with a single pump or with a chromatography system such as an ÄKTA™ system.

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