The HiScreen MabSelect SuRe column uses protein A chromatography for antibody purification. This protein A column is prepacked with MabSelect SuRe affinity resin and is part of our process development platform. These packed columns are used for method optimization and parameter screening for capture of monoclonal antibodies.

• The alkali-tolerant recombinant protein A ligand of MabSelect Sure allows clean-in-place (CIP).
• Prepacked, ready-to-use columns for convenient chromatography process development.
• 10 cm bed height of HiScreen columns is designed to allow method optimization and parameter screening.
• Easily connected in series to achieve 20 cm bed height.
• Small bed volume gives fast results and minimal sample/buffer consumption.
• Reproducible results, scalable to BioProcess columns packed with the same chromatography resin using the same linear fluid velocity.

The small column volume of 4.7 mL and bed height of 10 cm make HiScreen columns excellent tools for method optimization, parameter screening, robustness testing, and convenient scale-up of the protein purification process. Process fluid velocities can be applied, since the 10 cm bed height gives enough residence time and the results can then serve as basis for linear process scale-up.