DEAE Sepharose™ Fast Flow resin is a weak anion exchanger used for small-scale protein purifications and screening of binding/elution conditions.
HiTrap™ DEAE Sepharose™ Fast Flow column
Prepacked column for small-scale purification using weak anion exchange resin
HiTrap™ DEAE Sepharose™ Fast Flow column
Prepacked column for small-scale purification using weak anion exchange resin
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Overview
HiTrap DEAE FF is prepacked with DEAE Sepharose Fast Flow, and is a weak anion exchanger for small-scale protein purifications as well as screening of binding and elutions conditions.
- Convenient and affordable for fast, easy ion exchange separations either alone or connected in series.
- The industry standard for ion exchange chromatography.
- High flow rates and good scale-up potential.
- Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.
- Predictable scale-up
Q, SP, DEAE, and CM Sepharose Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities. ANX Sepharose 4 Fast Flow (high sub) is based on 4% highly cross-linked beaded agarose. This results in a resin with higher porosity, which is particularly useful for the purification of high molecular mass proteins.
The active end of the charged group is the same for DEAE Sepharose Fast Flow and ANX Sepharose Fast Flow (high sub), the difference is the length of the carbon chain of the charged group. DEAE Sepharose Fast Flow has a diethylaminoethyl-group bound to the agarose whilst ANX Sepharose 4 Fast Flow (high sub) has a diethylaminopropyl group attached.
Product specifications
Showing 2 products
HiTrap DEAE FF column, 5 x 1 ml
17505501
In stock7 available
HiTrap DEAE FF column, 5 x 5 ml
17515401
Selling fast5 available
Product availability is not guaranteed and subject to change.
