IMAC Sepharose High Performance resin

IMAC Sepharose High Performance is an uncharged IMAC resin for purifying proteins and peptides with an affinity for metal ions.

IMAC Sepharose High Performance and HiTrap IMAC HP

IMAC Sepharose High Performance resin

IMAC Sepharose High Performance is an uncharged IMAC resin for purifying proteins and peptides with an affinity for metal ions.

Pack size

25 mL

100 mL

IMAC Sepharose High Performance resin

To buy, choose your product.

Price includes standard shipping and handling

Overview

IMAC Sepharose High Performance is an uncharged IMAC resin for purifying proteins and peptides with an affinity for metal ions.

Supplied uncharged, for customized metal ion charging and optimized selectivity.
Suitable for optimizing purification of histidine-tagged (his-tagged) proteins when nickel is not the best choice of metal ion.
High performance purification due to its 34 μm bead size.
High binding capacity.

Uncharged resin for immobilized metal ion affinity chromatography

IMAC Sepharose High Performance is supplied free of metal ions, allowing a user to customize the charge to the target protein. Select a transition metal ion (e.g., Cu²⁺, Zn²⁺, Ni²⁺, or Co²⁺) that will bind to the covalently immobilized chelating ligand on the Sepharose.

This resin is commonly used for affinity purification of histidine-tagged proteins. However, it is also suitable for purifying native or untagged recombinant proteins.

Successful use in published research

IMAC Sepharose High Performance is trusted for protein purification in a wide body of research:

Structural analysis of an insect β-N-Acetyl-D-hexosaminidase
Evaluation of iron-binding properties of yeast extract hydrosylates

Compatible empty columns

Cytiva solutions include a variety of empty columns for packing. IMAC Sepharose High Performance resin is compatible for use with Tricorn, XK, and HiScale columns.