Melanie 9 Coverage software
Version 9.3.x - analyze how well your HCP antibody covers all the HCPs in your sample.
Melanie 9 Coverage software
Version 9.3.x - analyze how well your HCP antibody covers all the HCPs in your sample.
License type
Overview
Melanie Coverage is a comprehensive software solution for the host cell proteins (HCP) coverage assay with 2D differential in blot electrophoresis (2D DIBE) and other 2D gel electrophoresis images.
- Minimize false positives with an innovative 3D view
- Monitor the robustness of your assay with Coverage Range
- Reduce subjectivity with spot presence table
- Evaluate the performance of ELISA antibodies with automated quadrant coverage detection
- Run an accurate PDF report
- Choose between a node-locked license (a license fixed to one PC) or a floating license (a license that can be shared among PCs connected to a network license server)
HCP analysis dedicated software
Melanie Coverage is a tool for scientists involved in the development or use of immunoassays aimed at monitoring host cell protein (HCP) impurities in biopharmaceutical products. It is designed to analyze coverage, that is, the percentage of immunodetection that an antibody reagent offers for the total population of HCPs.
Comprehensive HCP analysis workflow support
The typical image pair used for coverage assessment consists of the primary image, representing the total protein content of the HCP-antigen, and the secondary image, which reveals the proteins detected with the anti-HCP antibody. These images are generated from a host of applications using 2D PAGE-based methods, notably:
- Conventional 2D gel electrophoresis followed by 2D Western blotting
- 2D Differential In Blot Electrophoresis (2D DIBE) CyDye based assay
- Antibody affinity chromatography followed by 2D DIGE
Simple workflow
Melanie Coverage guides you through the image analysis process with a user-friendly step-by-step workflow, delivering quick results.
- Quality Control
- Alignment
- Detection
- Spot edition
- Presence filter
- Coverage
Apply efficient alignment strategies
Melanie enables increased alignment efficiency and minimizes match editing work by defining an appropriate alignment strategy. This avoids aligning all images to a single reference that might be quite dissimilar to many gels in the data set and would generate an unnecessarily large number of difficult alignments.
Use free viewer functionality
Please download the Melanie Coverage analysis software package (200 MB) for Melanie. The software can open tutorial files and can be operated without licenses. To enable full functionality, contact us for a 21-day trial demo license.
Learn more about Cytiva's end-to-end solution for HCP analysis.
