Sephacryl™ High Resolution size exclusion chromatography resins
Available in five porosity ranges, suitable for fast, high recovery separations at laboratory and industrial scale
Sephacryl™ High Resolution size exclusion chromatography resins
Available in five porosity ranges, suitable for fast, high recovery separations at laboratory and industrial scale
1. Type
2. Volume
Overview
Sephacryl™ High Resolution size exclusion chromatography resins allow fast and reproducible purification of proteins, polysaccharides, and other macromolecules by size exclusion chromatography at laboratory and industrial scale.
- Purification over a wide molecular weight range
- High reproducibility due to high stability
- High recoveries
- Well-suited to industrial-scale use
The five chromatography resins available have different porosity ranges. For peptides and small proteins, Sephacryl S-100 HR is the best choice. When fractionating proteins in the molecular weight ranges of 5 × 103 to 2.5 × 105 and 1 × 104 to 1.5 × 106, use Sephacryl S-200 HR and Sephacryl S-300 HR, respectively. Note that these ranges include monoclonal antibodies and serum proteins.
Sephacryl S-400 HR and Sephacryl S-500 HR are recommended for separating polysaccharides and other macromolecules with extended structures, and even small particles such as plasmids.
Differentiating specs (see data file for full specifications):
| Application(s) | Sephacryl S-100 HR resin | Sephacryl S-200 HR resin | Sephacryl S-300 HR resin | Sephacryl S-400 HR resin | Sephacryl S-500 HR resin |
|---|---|---|---|---|---|
| Fractionation range (M*) | Peptides and small proteins | Antibodies, serum proteins and mid-size proteins | Plysaccharides, macromolecules with extended structures and even small particles such as plasmids | ||
| Globular proteins | ~ 1 × 103–~ 1 × 105 | ~ 5 × 103–~ 2.5 × 105 | ~ 1 × 104–~ 1.5 × 106 | ~ 2 × 104–~ 8 × 106 | -- |
| Dextrans | -- | ~ 1 × 103–~ 8 × 104 | ~ 2 × 103–~ 4 × 105 | ~ 1 × 104–~ 2 × 106 | ~ 4 × 104–~ 2 × 107 |
| Exclusion limit DNA (base pairs) | -- | ~ 30 bp | ~ 118 bp | ~ 271 bp | ~ 1078 bp |
Prepacked column options
All five Sephacryl HR resins are available in prepacked HiPrep™ Sephacryl HR SEC columns. Each chromatography resin is available in two different prepacked column sizes; 16/60 (120 mL) and 26/60 (320 mL). HiPrep Sephacryl HR SEC columns provide the excellent purification properties of Sephacryl HR resins in a convenient, ready-to-use format. Find the prepacked columns here.
