TALON® Superflow™ histidine-tagged protein purification resin
TALON® Superflow™ is a cobalt-based IMAC resin that offers a high-specificity alternative to nickel-charged resins for purifying histidine-tagged proteins.
TALON® Superflow™ histidine-tagged protein purification resin
TALON® Superflow™ is a cobalt-based IMAC resin that offers a high-specificity alternative to nickel-charged resins for purifying histidine-tagged proteins.
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Overview
TALON® Superflow™ is a cobalt-based IMAC resin that offers a high-specificity alternative to nickel-charged resins for purifying histidine-tagged proteins.
- High specificity – designed for histidine-tagged (his-tagged) purification of recombinant proteins when purity is more important than yield.
- Different selectivity compared to nickel-charged resins – an alternative to nickel affinity resins when a different metal ion is required.
High-specificity affinity resin for IMAC chromatography
TALON Superflow is used to purify his-tagged proteins from prokaryotic or eukaryotic expression systems. It is suitable for purification under native or denaturing conditions, and it can bind up to 20 mg of his-tagged protein per mL resin. The resin is compatible with commonly used IMAC reagents.
His-tagged proteins sometimes require cobalt-charged IMAC resin when nickel is not the optimal choice of metal ion. TALON Superflow is a high-quality option that offers different selectivity for affinity chromatography, typically providing protein with higher purity compared with nickel-based resins.
Successful use in published research
Applications for this his tag purification resin include:
- Purification of his-tagged antibody fragments to study Bothrops jararacussu venommyotoxicity
Convenient prepacked formats
TALON Superflow resin is available in the following prepacked formats:
- HiTrap TALON crude: HiTrap column format for convenient use with a syringe, a peristaltic pump, or a chromatography system
- His SpinTrap TALON: Spin columns for screening of small sample quantities using a benchtop centrifuge
- His GraviTrap TALON: gravity flow column for simple purification without the need for equipment
- His MultiTrap TALON 96-well plate: for high throughput screening and small scale purification using centrifuge or vacuum equipment
Product specifications
Showing 2 products
TALON Superflow, 50 mL
28957502
In stock8 available
TALON Superflow, 10 mL
28957499
Selling fast3 available
Product availability is not guaranteed and subject to change.
