Applications

For concentration of dilute sample volumes of up to 5 L
For use in diafiltration procedures which are faster and more efficient than dialysis for buffer exchange and desalting

FAQs

1 Q. How many times can a Minimate™ capsules be reused?
A. There are no specific guidelines for the total number of times a Minimate™ capsule can be reused as it is dependent on usage frequency, particles in the fluid, clean-in-place (CIP) procedure, normalized water permeability (NWP), and integrity testing. Pall recommends that a NWP value is taken on all new Minimate™ capsules prior use so after use and CIP, NWP can be re-evaluated. Typically, after CIP/regeneration the NWP of capsules should be >75% of the original NWP value, if it is not Pall recommends doing the cleaning procedure again. When the NWP value on a used Minimate™ capsule drops below 50% of the original NWP value, it is suggested that a new capsule is used.

2 Q. How many times can a Centramate™ cassettes be reused?
A. There are no specific guidelines for the total number of times a Centramate™ cassette can be reused as it is dependent on usage frequency, particles in the fluid, clean-in-place (CIP) procedure, normalized water permeability (NWP), and integrity testing. Pall recommends that a NWP value is taken on all new Centramate™ cassettes prior use so after use and CIP, NWP can be re-evaluated. Typically, after CIP/regeneration the NWP of cassettes should be >75% of the original NWP value, if it is not Pall recommends doing the cleaning procedure again. When the NWP value on a used Centramate™ cassette drops below 50% of the original NWP value, it is suggested that a new cassette is used.

3 Q. When should I order Centramate™ vs Centramate™ PE?
A. Certain applications require a fluid path that is free from metal. Our standard Centramate™ has stainless steel fittings and a 100% stainless steel cassette holder, so it would not be a good option for these applications. Pall’s Centramate™ PE has plastic fittings and the fluid path of the holder is polyethylene plastic. The pressure gauge that comes standard with the Centramate™ PE will have a stainless steel pathway, but the ¼” NPT fittings the system uses makes it easy to use a pressure gauge whose wettable parts will be free from metal.

4 Q. What do I need to attach an LV Centramate™ to the Minimate™ EVO system?
A. Pall recommends ordering the Minimate™ EVO Fittings kit, PN 97014, to get the additional 3-way stop cocks, hose barb adapters, and tubing needed to properly connect the LV Centramate™ holder to the Minimate™ EVO system. Contact your Pall representative or technical support if you have further questions.

5 Q. How many cassettes can I stack in a Centramate™ or Centramate™ PE?
A. You can stack up to 5 0.1 m2 T12 cassettes on Centramate™, giving you up to 0.5m2 of effective filtration area. For the Centramate™ PE, only 4 0.1 m2 T12 cassettes will fit because of the thicker PE base. Stacking multiple cassettes in the holders increases the surface area of the membrane which gives a higher permeate flow rate and increases the throughput.

6 Q. Guidance on average recirculation flow rates and permeate flux rates for the Minimate™, LV Centramate™, Centramate™ PE/SS.
A. For the Minimate™ we recommend a recirculation flow rate of 30-40 ml/min. If the inlet pressure is >30PSI at these flow rates, the rate should be decreased. A typical permeate flux rate will be 4 ml/min. For the LV Centramate™ we recommend a recirculation flow rate of 120-160 ml/min. If the inlet pressure is >30PSI at these flow rates, the rate should be decreased. A typical permeate flux rate will be 15 ml/min. For the Centramate™ and Centramate™ PE we recommend a recirculation flow rate of 600-800 ml/min. If the inlet pressure is >30PSI at these flow rates, the rate should be decreased. A typical permeate flux rate will be 75 ml/min. Note: These are typical values. Actual values may be higher or lower depending on the membr