MicroSpin Columns
Rapid purification of DNA for desalting DNA, buffer exchange, and removal of unincorporated nucleotides from end-labelled oligonucleotides.
Overview
Designed for the rapid purification of DNA, in conjunction with Sephadex G-25 DNA Grade or Sephadex G-50 DNA Grade, for desalting DNA, buffer exchange, and removal of unincorporated nucleotides from end-labelled oligonucleotides.
- For rapid buffer exchange/desalting of PCR products and other DNA samples in a volume of 10 to 100 µL using spin-column chromatography and Sephadex G-25 DNA Grade (not included)
- Excellent for rapid purification of newly synthesized oligonucleotides > 10-mers in 100 to 150 µL of deprotection solution using spin-column chromatography
- Flexible for your experiments, allowing differing amounts of Sephadex
- Can also be used for desalting/buffer exchange of DNA and removal of unincorporated radionucleotides from end-labeled oligonucleotides (at least 10 bases in length) in a volume of 10 to 100 µL
Sephadex G-25 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-25 has an exclusion limit of approximately Mr 5000, and with a spin protocol this means it can be used for any DNA greater than 10 bases in length.
Sephadex G-50 is a well-established gel filtration resin for desalting and buffer exchange of biomolecules > 30 000 molecular weight, and with a spin protocol can be used for DNA and oligo purification of molecules greater than 20 bases in length.
Both types of Sephadex are therefore highly suitable for the purification of oligonucleotides or very small DNA fragments following synthesis or a labeling reaction.
Sephadex G-25 DNA Grade and Sephadex G-50 DNA Grade are sold separately.
