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DoE for chromatography

What is design of experiments (DoE) and how to implement it for chromatography process development

Chromatography system illustration

What is DoE?

Design of experiments (DoE) is a technique for planning experiments and analyzing the information obtained. The technique allows you to use a minimum number of experiments. You systematically vary several experimental parameters simultaneously to obtain sufficient information to create a mathematical model of the chromatography process.

Design of experiments (DoE)

Improve process development efficiency compared to studying one factor at a time (OFAT)

A DoE model can be used to understand how experimental parameters affect outcomes. In chromatography process development, a DoE approach can greatly improve the efficiency in screening for suitable experimental conditions, in optimizing a process, or in robustness testing.