MabSelect PrismA™ protein A resin
Excellent productivity at commercial scale. Wide window of operation and well-suited for purification platforms.
Select protein A resin to optimize productivity in capturing mAbs
During clinical operation, resins are rarely used to their full lifetime. On the other hand, in large-scale manufacturing, a productive protein A chromatography step must run at high flow rates, using a high-capacity, high-durability resin. What should you consider for a cost-efficient protein A affinity chromatography step?
Evaluate binding sites
Affinity chromatography separates proteins based on a reversible binding interaction between the target protein and a ligand. Whether you’re purifying monoclonal antibodies or fragments such as Fabs or scFvs, knowing the binding site and structure of your target molecule is key to a successful purification. Click the figure to learn about protein A, protein L, and other affinity ligands.
MabSelect™ VL has a strong affinity to the variable region of antibody kappa light chains.
MabSelect PrismA™ resin, MabSelect PrismA™ X resin, and Fibro PrismA fiber units bind to both the VH3 and Fc regions. MabSelect™ SuRe 70 resin binds only to the Fc region.
MabSelect™ VH3 resin binds only to the VH region of the VH3 sequence family.
MabSelect PrismA™ resin, MabSelect PrismA™ X resin, and Fibro PrismA fiber units bind to both the VH3 and Fc regions.
MabSelect PrismA™ resin, MabSelect PrismA™ X resin, and Fibro PrismA fiber units bind to both the VH3 and Fc regions.
KappaSelect resin is designed for purification of human Fab (kappa) fragments. LambdaFabSelect resin binds to the constant region of the lambda light chain.
Capture
- MabSelect PrismA™ protein A chromatography resin
- MabSelect PrismA™ X protein A chromatography resin
- MabSelect™ SuRe 70 protein A chromatography resin
- HiScreen Fibro™ and HiTrap Fibro™ PrismA fiber chromatography units
- MabSelect™ VH3 protein A chromatography resin
- MabSelect™ VL protein L chromatography resin
- LambdaFabSelect chromatography resin
- KappaSelect chromatography resin
- Learn about all our affinity antibody resins
Polishing
- Capto™ Q ion exchange chromatography resin
- Capto™ S ImpAct ion exchange chromatography resin
- Capto™ adhere multimodal chromatography resin
- Capto™ adhere ImpRes multimodal chromatography resin
Columns
Systems
- ÄKTA™ chromatography systems for lab scale
- ÄKTA™ pilot 600 chromatography system
- ÄKTA™ ready chromatography system
Prepacked formats available
Our resins are available in multiple formats, from high-throughput process development tools to large-scale GMP manufacturing columns.
Process development
Affinity chromaography or mAb capture
- Capture of bispecific antibodies and removal of product-related impurities
- Protein L affinity resin is ready to move to manufacturing - comparing resin performance for mAb, Fab, and dAb samples
- Buffer optimization for separation of bsAb in a capture step using MabSelect™ VL resin
- Protein A affinity chromatography for one-step purification of a bispecific antibody in early screening
- Optimization of a two-step purification method for bispecific antibodies
- Toolbox approaches for antibody fragment purification
- A high-throughput study of sodium hydroxide cleaning efficiency for protein A resin
- Influence of NaOH concentration on protein A resin cleaning
- Register for print resin selection poster
- Process intensifier app
- Podcast: The discovery of protein A
- Podcast: Get the Most Out of Protein A Capture
- Webcast: Advancements in Antibody Purification
- White paper: Purification of monoclonal antibodies using modern chromatography media and membranes
- Application note: Capacity and performance of MabSelect PrismA™ protein A chromatography resin
- Application note: Lifetime performance study of MabSelect PrismA™ during repeated cleaning-in-place cycles
- Application note: Optimizing productivity on high-capacity protein A chromatography resins
- White paper: Efficient cleaning-in-place methods for protein-based antibody affinity chromatography resins
- Application note: Evaluation of protein A resin lifetime during extensive use (overloading) in continuous chromatography mode
- Application note: Process economy simulation of mAb capture step with MabSelect PrismA™ protein A chromatography resin
- Application Note: Multidimensional scale-up of a monoclonal antibody capture step, using ÄKTA™ pilot 600
- Case study: Efficient protein A chromatography for bispecific antibodies
- Article: Large-scale column packing for MabSelect PrismA™ resin