Column-based
Nucleic acid
Extraction kits
Kits to extract plasmids, genomic DNA, and RNA from liquid biopsy, buffy coat, cultured mammalian cells, and solid tissue samples.
Frequently asked questions
DNA and RNA extraction
DNA and RNA are two types of nucleic acids that play critical roles in living organisms. DNA contains genetic information, and RNA is responsible for translating this genetic information into proteins.
DNA and RNA extraction processes are used to isolate and purify nucleic acids from samples, such as cultured cells or tissue samples. The purpose of these extraction methods is to obtain high-quality DNA or RNA that can be used for various downstream applications such as PCR, quantitative PCR (qPCR), next-generation sequencing (NGS), gene expression analysis, and genotyping.
Nucleic acid extraction typically involve lysis, or breaking open the cells, with methods such as mechanical or chemical disruption or enzymatic digestion. Lysis is followed by steps to release the nucleic acids and remove impurities or contaminants. These steps can include separation and purification of the nucleic acids by precipitation, column chromatography, or magnetic bead-based separation. The extraction method you should use depends on factors such as sample type, the purity and yield required, and your downstream applications.
We offer extraction kits for plasmid DNA, genomic DNA, and RNA, as well as for simultaneous extraction and isolation of genomic DNA, total RNA, and total protein from animal tissues and mammalian cells.
Genomic DNA extraction kits
Genomic DNA (gDNA) extraction kits isolate and purify gDNA from samples such as blood, tissues, or cells. These kits provide high-quality gDNA for downstream applications such as PCR, qPCR, NGS, genotyping, and cloning.
Plasmid DNA extraction kits
Plasmid DNA kits enable researchers to obtain high-quality plasmid DNA for various applications, such as cloning, transformation, gene expression analysis, and DNA sequencing. The mechanism of adding a DNA plasmid into a mammalian cell is known as plasmid transfection, and for it to work, transfection-grade plasmid DNA is needed. To obtain efficient gene transfer by transfection, plasmid DNA can be complexed with lipid reagents for more efficient delivery into the cell's nucleus, essential for subsequent protein expression of the gene of interest.
RNA extraction kits
RNA extraction is the purification of RNA from biological samples. It is complicated by the ubiquitous presence of ribonuclease enzymes in cells and tissues, which can rapidly degrade RNA, since it is less stable than DNA. Besides other methods in the RNA extraction kit protocol to help counter RNA degradation, the equipment used for RNA preparation is usually kept separate from other lab equipment and treated (or has been pre-treated) with chemicals that can destroy RNases, such as diethyl pyrocarbonate (DEPC). Our RNA extraction kits can be used for fast and efficient total RNA extraction from cultured cells, tissue, bacteria or yeast. Purified RNA can be used in downstream applications such as quantitative reverse transcription PCR (qRT-PCR), reverse transcription PCR (RT-PCR), gene expression profiling, primer extension, and RNase protection assays.
Total gDNA/RNA/protein extraction kits
Total gDNA/RNA/protein extraction kits are designed to simultaneously isolate and purify genomic DNA, RNA, and proteins from a single biological sample. Our TriplePrep Kit is designed for rapid, simultaneous extraction and isolation of high-quality genomic DNA (gDNA), total RNA, and total denatured proteins from animal tissues and mammalian cells. This enables the correlation of gene structure, gene expression, and protein expression, with efficient extraction of DNA, RNA, and protein from one and the same sample.