PlasmidPrep Midi Flow Kit
Designed for the purification of high yields of transfection-grade plasmid DNA that are proportional to the input sample volume.
PlasmidPrep Midi Flow Kit
Designed for the purification of high yields of transfection-grade plasmid DNA that are proportional to the input sample volume.
Pack size
Overview
PlasmidPrep Midi Flow Kit is a gravity flow plasmid prep kit designed for the purification of large yields of transfection-grade plasmid DNA that is proportional to the input sample volume.
- Provides plasmid DNA with excellent purity, > 70% (strain-dependent) of supercoiled plasmid DNA
- Increased ease of use due to minimal pipetting volume changes, color-coded kit components, and pre-equilibrated columns
- Consistently produces pure plasmid DNA with low levels of endotoxin contamination, < 10 endotoxin units (EU) per μg of plasmid DNA
The mechanism of adding a DNA plasmid into a mammalian cell is known as plasmid transfection, and for it to work, transfection-grade plasmid DNA is needed. To obtain efficient gene transfer by transfection, plasmid DNA can be complexed with lipid reagents for more efficient delivery into the cell's nucleus, essential for subsequent protein expression of the gene of interest.
PlasmidPrep Midi Flow Kit can be used to process bacterial sample volumes of 25 to 50 mL for high copy number plasmid DNA and up to 500 mL for low copy number plasmid DNA. The kit consists of a high-performance, anion exchange purification medium that combines excellent flow characteristics with an exceptional capacity and specificity for plasmid DNA.
PlasmidPrep Midi Flow Kit contains 250 fast-flow plasmid columns, resuspension, lysis, neutralization, wash, elution, and Tris-EDTA (TE) buffers, and a full protocol booklet with a detachable, quick reference protocol card.
