Biacore™ cap-tag capture kit
Capture kit enables kinetic and affinity analysis of protein-protein interactions using Biacore cap-tag conjugation
For use with
Overview
Biacore™ cap-tag capture kit simplifies kinetic and affinity analysis of protein interactions with minimal assay development. Attach your protein to Series S Sensor Chip CAP using a Biacore™ cap-tag, enabling reversible capture and easy regeneration for up to 150 cycles.
- Simple protein capture regardless of existing tag
- Save time and effort. No need to develop attachment or regeneration conditions.
- A reusable and cost-effective solution for protein interaction analysis
- Easy-to-follow instructions and predefined Biacore™ Insight software methods for your convenience
This capture kit enables attachment of your target protein to a Biacore™ sensor chip by first conjugating a short oligonucleotide tag (Biacore™ cap-tag) to your protein. Biacore™ cap-tag binds to the complementary oligo on Series S Sensor Chip CAP.
The resulting Biacore™ cap-tag protein conjugate can be reversibly captured for protein interaction analysis in Biacore™ systems. After each analysis cycle, the surface is regenerated by removing the protein and any bound analyte, which allows fresh protein conjugate to be attached in subsequent cycles.
Biacore cap-tag capture kit series s is sold as a combination of three different products containing all reagents required for conjugation and capture of Biacore cap-tag ligand conjugates:
- Biacore™ cap-tag conjugation kit that contains Biacore™ conjugation buffer, Biacore™ ligand activator, and Biacore™ cap-tag
- Amersham™ MicroSpin™ G-50 columns for buffer exchange and purification
- Series S Sensor Chip CAP
The capture kit should be used alongside:
- Biacore™ regeneration kit CAP, which contains stocks for preparing regeneration solution for Series S Sensor Chip CAP
Please note:
- If ordering Biacore™ cap-tag capture kit series s you will also need to order Biacore™ regeneration kit CAP separately to regenerate your sensor chip.
- All products included in Biacore™ cap-tag capture kit can be purchased as standalone items, which provide flexibility for your assay set up. If you already have solutions or sensor chips, you can purchase any additional products needed below.
- Biacore™ cap-tag conjugation kit and Biacore™ regeneration kit CAP can only be purchased on this page. You can add to cart in the production specification section below.
- Amersham™ MicroSpin™ G-50 columns for buffer exchange and purification and Series S Sensor Chip CAP can be purchased on their standalone pages.
Comprehensive and detailed instructions guide you through the complete workflow from protein to evaluated kinetic data. We provide predefined run and evaluation methods for Biacore™ Insight software for added convenience.
Biacore™ cap-tag capture kit series s is developed specifically for protein-protein interaction studies on Biacore™ 8 series, Biacore™ 1 series, Biacore™ T200, and Biacore™ S200 SPR systems, but can be used for other applications.
Biacore™ cap-tag conjugation kit and Biacore™ regeneration kit CAP can be used with any Biacore™ system, but Biacore™ X100 and Biacore™ legacy system users will need to combine these with Sensor Chip CAP which is a component of Biotin CAPture kit (28920233).
The kit contains deoxyribo-oligonucleotides, which makes it unsuitable for use with DNA-binding proteins or enzymes that degrade DNA.
Note: Certificate of conformance is available for Biacore™ cap-tag conjugation kit and Biacore™ regeneration kit CAP, but no certificate of analysis is provided.
