PreDictor™ RoboColumn™ Ni Sepharose™ 6 Fast Flow
Prepacked minicolumns with Ni Sepharose™ 6 Fast Flow resin for HTPD of purification of his-tagged proteins.
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Overview
These PreDictor™ RoboColumn™ minicolumns for high-throughput process development (HTPD) are prepacked with Ni Sepharose™ 6 Fast Flow resin for purification of histidine-tagged (his-tagged) proteins.
- Supports high-throughput process development (HTPD) — enables parallel screening of chromatographic conditions using a miniaturized column format and robotic workstation.
- Miniaturized column format — facilitates screening of a wide range of parameters with small sample consumption.
- Reduced experimental time — automation and parallel screening minimize labor and save time.
- Increased process understanding — high-throughput workflow allows investigation of enlarged experimental workspace for better process understanding.
- Packed with Ni Sepharose™ 6 Fast Flow resin — for purification of his-tagged proteins.
Ni Sepharose™ 6 Fast Flow IMAC resin
Ni Sepharose™ 6 Fast Flow immobilized metal affinity chromatography (IMAC) resin is designed for purification of his-tagged proteins. It is based on cross-linked agarose beads modified with a chelating ligand immobilized to the base matrix and precharged with nickel ions.
Ni Sepharose™ 6 Fast Flow resin meets industrial demands with security of supply, robust performance, and regulatory support.
Learn more about Ni Sepharose™ Fast Flow resin.
PreDictor™ RoboColumn™ units for HTPD
PreDictor™ RoboColumn™ units are prepacked, miniaturized columns that support HTPD using a robotic liquid handling workstation for fully automated and parallel chromatographic separations. HTPD work can be performed using PreDictor™ RoboColumn™ units alone, or as a complement to PreDictor™ 96-well filter plates. PreDictor™ RoboColumn™ units can be operated in true chromatography mode, enabling dynamic binding capacity studies, while 96-well plates enable the study of static binding capacities.
PreDictor™ RoboColumn™ units are supplied in rows of eight columns with the same chromatography resin, and can be individually and easily arranged on an array plate containing 96 positions.
* RoboColumn is a trademark of Repligen GmbH.
