PreDictor™ RoboColumn™ Ni Sepharose™ excel
Prepacked IMAC chromatography minicolumns for HTPD of his-tagged protein purification. Suitable for samples containing nickel stripping agents.
PreDictor™ RoboColumn™ Ni Sepharose™ excel
Prepacked IMAC chromatography minicolumns for HTPD of his-tagged protein purification. Suitable for samples containing nickel stripping agents.
Bed volume
Overview
These PreDictor™ RoboColumn™ minicolumns for high-throughput process development (HTPD) are prepacked with Ni Sepharose™ excel affinity resin. For capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).
- Supports HTPD — enables parallel screening of chromatographic conditions using a miniaturized column format and robotic workstation.
- Miniaturized column format — facilitates screening of a wide range of parameters with small sample consumption.
- Reduced experimental time — automation and parallel screening minimize labor and save time.
- Increased process understanding — high-throughput workflow allows investigation of enlarged experimental workspace for better process understanding.
- Packed with Ni Sepharose™ excel resin — designed for his-tagged protein purification with minimized Ni-leakage and maximized protein recovery when samples contain stripping agents.
Ni Sepharose™ excel IMAC resin
Ni Sepharose™ excel resin is designed for purification of his-tagged proteins when nickel leakage is an issue. Minimize Ni-leakage and maximize protein recovery in his-tag protein purification, even when samples contain nickel stripping agents.
The resin flow properties make it suitable for purifications in a wide range of scales. You can load large sample volumes, enabling purification of target proteins at low concentrations.
Learn more about Ni Sepharose™ excel IMAC resin.
PreDictor™ RoboColumn™ units for HTPD
PreDictor™ RoboColumn™ units are prepacked, miniaturized columns that support HTPD using a robotic liquid handling workstation for fully automated and parallel chromatographic separations. HTPD work can be performed using PreDictor™ RoboColumn™ units alone, or as a complement to PreDictor™ 96-well filter plates. PreDictor™ RoboColumn™ units can be operated in true chromatography mode, enabling dynamic binding capacity studies, while 96-well plates enable the study of static binding capacities.
PreDictor™ RoboColumn™ units are supplied in rows of eight columns with the same chromatography resin, and can be individually and easily arranged on an array plate containing 96 positions.
* RoboColumn is a trademark of Repligen GmbH.
