Ready-To-Go DNA Labeling Beads (-dCTP)
Ready-To-Go DNA Labeling Beads (-dCTP)
Ready-To-Go DNA Labeling Beads (-dCTP)
Ready-To-Go DNA Labeling Beads (-dCTP)
Overview
- Ambient-temperature-stable, single-dose reaction spheres for labeling DNA to specific activities > 10⁹ dpm/µg in as little as 5 min using labeled dCTP.
- Predispensed spheres in individual reaction tubes eliminate the potential for cross-contamination.
- Beads reconstitute immediately upon addition of water or buffer to a final reaction volume of 50 µl.
- DNA can be labeled in solution or in the presence of low melting point agarose.
- Reaction mixtures efficiently incorporate 32P- or 35S-labeled dCTP.
The kit includes the following reagents, sufficient for 20 reactions using 32P- or 35S-labeled dCTP. Reaction mix: 20 individual reaction spheres glassified from a buffered aqueous solution containing dATP, dGTP, dTTP, random nonamers [d(N)₉] and Klenow fragment, and control DNA.
Ready-To-Go DNA Labeling Beads (-dCTP) provide a rapid, reliable means of labeling DNA using labeled dCTP or analogs. The components in the kit are provided in a room-temperature-stable format; each Reaction Mix comes as a dried bead and is ready for immediate use in “oligolabelling”. Each reaction is capable of labeling between 10 ng and 1 µg linearized DNA. Using 25 to 50 ng DNA and [α-32P]dCTP at 3000 Ci/mmol, a specific activity of >1 x 10⁹ dpm/µg can be achieved in as little as 5 to 15 min.
