Superdex™ 75 Increase prepacked columns are designed for rapid separation and analysis of proteins by size exclusion chromatography (SEC), also called gel filtration. They can be used for applications such as preparative protein purification, aggregate analysis, studies of complex formation, and screening of samples.
Superdex™ 75 Increase columns
Prepacked size-exclusion chromatography columns for separation and analysis of proteins with molecular weights ranging from Mr 3000 to 70 000
Superdex™ 75 Increase columns
Prepacked size-exclusion chromatography columns for separation and analysis of proteins with molecular weights ranging from Mr 3000 to 70 000
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Bed Dimensions
Superdex™ 75 Increase column, 10/300 GL
29148721
In stock–22 available
Price includes standard shipping and handling
Overview
Superdex™ 75 Increase 10/300 GL, 5/150 GL, and 3.2/300 are prepacked columns for versatile use in size exclusion/gel filtration chromatography applications. The columns are intended for use in preparative purification as well as for characterization and analysis of proteins with molecular weights between Mr 3000 and 70 000, such as recombinant tagged proteins.
- Up to 50% higher resolution compared with predecessor Superdex™ 75, for improved purity and analysis results.
- Runtime reduced down to one third compared with predecessor Superdex™ 75, with maintained resolution for results faster.
- Versatile use in both preparative and analytical applications, especially with recombinant tagged proteins.
- Higher lot-to-lot consistency compared with predecessor Superdex™ 75.
- Tolerance to repeated harsh cleaning procedures at high pH ensures long column lifetime and minimizes carryover.
Higher resolution and/or reduced runtime compared with predecessor
Superdex™ 75 Increase belongs to the new generation of agarose based SEC media, comprising smaller, more rigid beads with a narrower particle size distribution and a higher selectivity for improved performance compared with its predecessor Superdex™ 75.
Clean in place for versatile use
Superdex™ 75 Increase resin is alkali-resistant. Hence, the column can be cleaned in place under alkali conditions to allow use of the same column for different proteins with minimal risk for carryover between samples. The alkali resistance is what differentiates agarose-based resins from silica-based resins. The possibility to clean the resin under harsh alkali conditions also ensures a long column lifespan.
Product specifications
Showing 3 products
Superdex™ 75 Increase column, 10/300 GL
29148721
In stock22 available
Superdex™ 75 Increase column, 5/150 GL
29148722
In stock9 available
Superdex™ 75 Increase column, 3.2/300
29148723
In stock9 available
Product availability is not guaranteed and subject to change.
