Fractionation of large proteins and protein complexes

Suitable for both preparative and analytical applications, Superose™ 6 Increase SEC columns are the first choice for the characterization of large proteins and protein complexes. They can also be used the study of protein-protein interactions, and as a screening tool to explore the molecular weight distribution of an unknown sample.

Superose™ 6 and Superdex™ 200 analysis ranges are complementary

Superose™ 6 Increase is designed for separation of protein complexes and other very large molecules, including those with molecular weights exceeding M_r ^~400 000. The optimal fractionation range of Superdex™ 200 Increase is complementary, providing higher resolution for proteins with molecular weights below M_r ^~400 000.

Cleaning-in-place (CIP) capabilities

This agarose-based resin is alkali resistant and supports cleaning-in-place (CIP) procedures. This capability allows the same column to be used for different proteins, with minimal risk of carry-over between samples.

Regular cleaning can also increase column lifetime.

Choose from 3 column sizes to suit varying application needs

Feedback on Superose™ 6 Increase SEC columns

"Now I can work on more samples, resulting in higher throughput. I can now purify a protein in 30 minutes instead of an hour." Dr. Tomohiro Nishizawa (Nureki Laboratory). Department of Biological Sciences, Graduate School of Science, The University of Tokyo

Successful use in published research

Researchers use these pre-packed columns for a variety of applications, such as:

• Study of a magnetic sensing protein
• Evaluation of effects two proteins have on microtubule nucleation
• Production of virus-like particles in E. coli