Enjoy Free Shipping on most orders placed online – Terms Apply

Superose 6 Increase small-scale SEC columns

Size exclusion chromatography columns: for high-resolution protein purification and analysis in MW range Mr~ 5K to ~ 5M.

Order Product

ID	Product Name
29091596	Superose 6 Increase 10/300 GL
29091598	Superose 6 Increase 3.2/300
29091597	Superose 6 Increase 5/150 GL

Superose 6 Increase SEC columns are designed for high-resolution, small-scale preparative purification, and analysis of large globular proteins, protein complexes, and other macromolecules in the molecular weight range M_r^~ 5K to ^~ 5M.

Wide fractionation range: optimized to purify large biomolecules, including membrane proteins, protein complexes, and DNA fragments
Very high resolution: small bead size and a narrow particle size distribution provide high resolution, for high protein purity
High flow rates: rigid beads give excellent pressure/flow properties
Enhanced performance: improved resolution and runtime compared to predecessor

“Increase”: Cytiva's new generation of size exclusion chromatography columns

Superose 6 Increase is a next-generation, agarose-based matrix for size exclusion chromatography. With smaller and more rigid beads than their predecessors, Superose 6 Increase columns deliver higher resolution protein purification in shorter runtimes.

Fractionation of large proteins and protein complexes

Suitable for both preparative and analytical applications, Superose 6 Increase SEC columns are the first choice for the characterization of large proteins and protein complexes. They can also be used the study of protein-protein interactions, and as a screening tool to explore the molecular weight distribution of an unknown sample.

Superose 6 and Superdex 200 analysis ranges are complementary

Superose 6 Increase is designed for separation of protein complexes and other very large molecules, including those with molecular weights exceeding M_r ^~400 000. The optimal fractionation range of Superdex 200 Increase is complementary, providing higher resolution for proteins with molecular weights below M_r ^~400 000.

Cleaning-in-place (CIP) capabilities

This agarose-based resin is alkali resistant and supports cleaning-in-place (CIP) procedures. This capability allows the same column to be used for different proteins, with minimal risk of carry-over between samples.

Regular cleaning can also increase column lifetime.

Choose from 3 column sizes to suit varying application needs

Column	Recommended sample volume	Recommended operating flow	Efficiency (N/m)	Applications
Superose 6 Increase 3.2/300	4 to 50 µL	0.04 mL/min	>48,000	Small-scale preparative purification and analysis of large proteins and other biomolecules, when small sample and buffer consumption is important
Superose 6 Increase 10/300 GL	25 to 500 µL	0.5 mL/min	> 48,000	Standard for small-scale preparative purification and analysis of large proteins and other biomolecules, especially protein complexes
Superose 6 Increase 5/150 GL	4 to 50 µL	0.3 mL/min	> 42,000	Rapid purity check and homogeneity analysis of large proteins and protein complexes

Feedback on Superose 6 Increase SEC columns

"Now I can work on more samples, resulting in higher throughput. I can now purify a protein in 30 minutes instead of an hour." Dr. Tomohiro Nishizawa (Nureki Laboratory). Department of Biological Sciences, Graduate School of Science, The University of Tokyo

Successful use in published research

Researchers use these pre-packed columns for a variety of applications, such as:

Study of a magnetic sensing protein
Evaluation of effects two proteins have on microtubule nucleation
Production of virus-like particles in E. coli

Read less

Select your model

Parameter	{{main.activeVariantName}}
There's no available product features to display for this product variant.
^{{{main.calculateFootnoteIndex(feature, main.features.ownFeatures)}}}

^{{{main.calculateFootnoteIndex(feature, main.features.ownFeatures)}}}{{feature.footnote}}

Parameter	{{referenceSection.referenceName}}
There's no available product features to display for this product variant.
^{{{main.calculateFootnoteIndex(feature, referenceSection.featureValues)}}}

^{{{main.calculateFootnoteIndex(feature, referenceSection.featureValues)}}}{{feature.footnote}}

Something went wrong - we were not able to load your agreed MSA pricing. Please try refreshing the page.

Product	Product Name	Bed Dimensions All 3.2 × 300 mm 5 x 150 mm 10 x 300	Price
29091596	Superose 6 Increase 10/300 GL	10 x 300	2 043.00 USD	Add to cart Get Quote In Stock Stock on request Limited stock
29091597	Superose 6 Increase 5/150 GL	5 x 150 mm	2 027.00 USD	Add to cart Get Quote In Stock Stock on request Limited stock
29091598	Superose 6 Increase 3.2/300	3.2 × 300 mm	2 449.00 USD	Add to cart Get Quote In Stock Stock on request Limited stock

Superose 6 Increase small-scale SEC columns

Superose 6 Increase small-scale SEC columns

“Increase”: Cytiva's new generation of size exclusion chromatography columns

Fractionation of large proteins and protein complexes

Superose 6 and Superdex 200 analysis ranges are complementary

Cleaning-in-place (CIP) capabilities

Choose from 3 column sizes to suit varying application needs

Feedback on Superose 6 Increase SEC columns

Successful use in published research

Refine

Customers also bought

Leave a Review

Product Support

Looking for support assistance?

Superose 6 Increase small-scale SEC columns

Superose 6 Increase small-scale SEC columns

“Increase”: Cytiva's new generation of size exclusion chromatography columns

Fractionation of large proteins and protein complexes

Superose 6 and Superdex 200 analysis ranges are complementary

Cleaning-in-place (CIP) capabilities

Choose from 3 column sizes to suit varying application needs

Feedback on Superose 6 Increase SEC columns

Successful use in published research

Select your model

{{referenceSection.sectionTitle}}

Refine

Customers also bought

Leave a Review

Product Support

Looking for support assistance?