The high binding capacity and affinity to a broad range of antibody fragments makes this resin an excellent capture platform for antibody fragments containing kappa light chains. Common uses include domain antibody (dAb), scFv, and Fab purification.

Using this protein L resin

Capto™ L resin requires minimal optimization and produces high purity in one separation step, resulting in significant savings during process development. This resin is also advantageous in manufacturing, because high selectivity of affinity capture reduces the number of subsequent polishing steps.

Streamlined scale up

Scale-up with this resin is straightforward and is further aided by a range of prepacked process development tools, such as PreDictor™ plates, PreDictor™ RoboColumn™, and HiScreen™ prepacked columns.

Successful use in published research

Capto™ L is used in applications requiring the purification of antibody fragments through protein L affinity chromatography.

Compatible empty columns – research scale

Capto™ L resin is compatible with empty PD gravity flow, Tricorn™, XK, and HiScale™ columns.

Compatible empty columns – bioprocess scale

For process-scale applications, Cytiva recommends AxiChrom™ columns.

Convenient prepacked columns – research scale

Capto™ L resin is available in the following formats:

• HiTrap™ Protein L prepacked columns: convenient for easy use with a syringe, peristaltic pump, or chromatography system
• HiScreen™ Capto™ L prepacked columns, for method and process development
• PreDictor™ Capto™ L 96-well plates, for high-throughput screening or process development

Convenient prepacked columns – bioprocess scale

Prepacked ReadyToProcess™ columns are available, eliminating the need for column packing and cleaning validation.