Besides other methods in the RNA isolation protocol to help counter RNA degradation, the equipment used for RNA preparation is usually kept separate from other lab equipment and treated (or has been pre-treated) with chemicals that can destroy RNases, such as diethyl pyrocarbonate (DEPC). RNAspin Mini Isolation Kit can be used to isolate total RNA from cultured cells, tissue, bacteria, or yeast. The isolated RNA is of a high enough quality and quantity (up to 100 μg) for multiple downstream experiments and sensitive enzymatic applications like RT-PCR, real time PCR, primer extension, RNAseq, and RNase protection assays.
The RNAspin Mini Kit has been used for the isolation of high-quality total RNA from several types of source material including hard-to-lyse bacterial strains and can be applied in downstream experiments such as quantitative reverse transcriptase PCR (QRT-PCR), Northern blot, and microarray experiments. The kit is supplied with a full RNA extraction protocol booklet with a detachable, quick reference protocol card, and all the necessary components including prefilters and DNase I.