FAQ

Spare parts

Location of the I/O box

The illustration below shows the I/O-box and its recommended location and connection points.

 

Function of the I/O-box

The I/O-box E9 is used to interface other equipment in order to measure parameters such as refractive index, light scattering and fluorescence. See Requirements on connected equipment, on page 196 for information on requirements of the equipment that can be connected to ÄKTA pure. The I/O-box can control external equipment by a digital output signal, as well as detecting the state of them by digital inputs. It is possible to install up to two I/O-box E9 when using ÄKTA pure. If two I/O-boxes are to be used, the second one has to be configured as I/O-box E9, 2nd. The configuration is defined by the I/O-box E9 Node ID.

Part Description
1 I/O-box
2 D-sub connectors

 

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# Product Name Product Code Price
I/O-box E9 29011361 2 377.00 USD Add to cart
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# Product Name Product Code Price
1 Module Panel 29011364 171.46 USD Add to cart
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2 Multi-module panel 29011351 609.00 USD Add to cart
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# Product Name Product Code Price
1 Jumper 1 IEC 1394 28956489 45.29 USD Add to cart
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2 Jumper D-Sub 29011365 157.13 USD Add to cart
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4 External module cable 29011366 145.29 USD Add to cart
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5 Cable 2.5m UniNet-9 D-type 29032425 117.54 USD Add to cart
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# Product Name Product Code Price
Inlet filter holder kit 11000407 140.19 USD Add to cart
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Inlet filter set 11000414 91.66 USD Add to cart
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Tubing cutter, for PEEK, EFTE, and FEP tubing i.d. 0.25, 0.5, 0.75, 1 and 1.6 mm 18111246 80.74 USD Add to cart
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Reference capillary 1 28950749 111.09 USD Add to cart
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Reference capillary 2 28950750 111.09 USD Add to cart
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Union 1/16 male/male, i.d. 0.5 mm 28954326 137.76 USD Add to cart
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ETFE Tubing Kit 10 x 1.0 m, i.d. 1.0 mm, o.d. 1/16" 28980995 168.97 USD Add to cart
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Tubing Kit, 0.50 mm 29011327 956.00 USD Add to cart
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Tubing Kit, 0.25 mm 29011328 958.00 USD Add to cart
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Tubing Kit, 0.75 mm 29011329 956.00 USD Add to cart
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Tubing kit for inlet valve V9-IAB 29011330 249.00 USD Add to cart
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Tubing kit for V9-pH, standard 29011331 238.00 USD Add to cart
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Tubing kit for V9-IA (7-ports) 29011332 259.00 USD Add to cart
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Tubing kit for V9-IB (7-ports) 29011333 258.00 USD Add to cart
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Tubing kit for outlet fractionation (10 outlets) 29011334 232.00 USD Add to cart
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System Pump Rinse Tubing Kit 29011348 117.54 USD Add to cart
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Tubing Kit i.d. 1.0 mm 29032426 976.00 USD Add to cart
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Tubing kit for Sample Inlet Valve V9-IS 29035331 258.00 USD Add to cart
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System Tubing kit for ÄKTA pure 150, i.d. 0.75 mm 29048242 958.00 USD Add to cart
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Tubing Kit for Outlet Fractionation (10 outlets) 29048611 232.00 USD Add to cart
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Tubing kit for Sample Inlet Valve V9H-IS 29051166 258.00 USD Add to cart
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Tubing kit for V9H-IB (7-ports) 29051189 258.00 USD Add to cart
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Tubing kit for V9H-IA (7-ports) 29051197 258.00 USD Add to cart
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System Tubing kit for ÄKTA pure 150, i.d. 0.50 mm 29051669 956.00 USD Add to cart
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V9H-pH Tubing Kit 29051674 231.00 USD Add to cart
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Location and illustration of pumps

The illustration below shows the location of System pump A and System pump B, together with a detailed view of a system pump. System pump A is labeled P9 A and System pump B is labeled P9 B.

Part Description
1 Purge valve: Used to remove air from the pump
2 Outlet port with check valve
3 Connections to pump piston rinsing system: Tubing is connected between the pumps and the Pump piston rinsing system tube (6)
4 Inlet port with check valve
5 Pump head: Encapsulates the inner parts of the pump
6 Pump piston rinsing system tube
7 System pump B
8 System pump A

 

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# Product Name Product Code Price
Check valve kit 18112866 1 134.00 USD Add to cart
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Piston kit, 25 ml 28952640 758.00 USD Add to cart
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P9 Seal kit, 25 ml 28952642 562.00 USD Add to cart
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P9H Check valve kit 28979364 925.00 USD Add to cart
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P9H Piston kit 150 ml 28979368 740.00 USD Add to cart
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P9H Seal kit 150 ml 28979373 591.00 USD Add to cart
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Sample Pump S9H 29050593 7 812.00 USD Add to cart
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Typical configuration of an ÄKTA pure 25 (25 ml/min) system.

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# Product Name Product Code Price
1 Inlet Valve Kit V9-IA 29012263 5 477.00 USD Add to cart
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3 Check valve kit 18112866 1 134.00 USD Add to cart
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3 P9 Seal kit, 25 ml 28952642 562.00 USD Add to cart
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5 Check valve kit 18112866 1 134.00 USD Add to cart
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5 Piston kit, 25 ml 28952640 758.00 USD Add to cart
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5 P9 Seal kit, 25 ml 28952642 562.00 USD Add to cart
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6 Online filter kit 18102711 120.78 USD Add to cart
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6 O-ring 13.1x1.6 mm 28953545 70.52 USD Add to cart
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6 Mixer chamber 0.6 ml 28956186 1 134.00 USD Add to cart
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6 Mixer chamber 1.4 ml 28956225 1 146.00 USD Add to cart
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6 Mixer chamber 5 ml 28956246 1 146.00 USD Add to cart
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6 O-ring 13.1 x 1.6 mm High resistance 29011326 171.12 USD Add to cart
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6 Outlet Valve Kit V9-Os 29011356 2 117.00 USD Add to cart
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7 Outlet valve kit V9-O 29012261 3 610.00 USD Add to cart
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8 Injection Valve V9-Inj 28956514 3 404.00 USD Add to cart
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9 Conductivity monitor (C9n) 29011363 2 434.00 USD Add to cart
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10 Column Valve V9-Cs 29011355 3 111.00 USD Add to cart
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10 Column Valve kit V9-C 29011367 7 342.00 USD Add to cart
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11 UV flow cell, 5 mm, for U9-L 18112824 2 166.00 USD Add to cart
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11 UV flow cell, 10 mm, for U9-M 28956378 1 987.00 USD Add to cart
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11 UV flow cell, 2 mm, for U9-M 28979380 2 954.00 USD Add to cart
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11 UV flow cell, 0.5 mm, for U9-M 28979386 3 067.00 USD Add to cart
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11 UV flow cell, 2 mm, for U9-L 29011325 2 372.00 USD Add to cart
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11 UV Monitor U9-L 29011360 4 994.00 USD Add to cart
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12 Multi-module panel 29011351 609.00 USD Add to cart
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13 Tube Rack 40 × 30 mm, bowl, tube support, holder and guide 18112467 509.00 USD Add to cart
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13 Tube Holder and Guide, 40 × 30 mm 18112468 423.00 USD Add to cart
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13 Tube Rack Complete, 95 × 10–18 mm 18305003 570.00 USD Add to cart
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13 Bowl 18305103 166.52 USD Add to cart
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13 Tube Support 18305402 129.15 USD Add to cart
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13 Tubing Holder 18646401 83.03 USD Add to cart
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13 Drive sleeve 19606702 108.13 USD Add to cart
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13 Tube Holder and Guide, 175 × 12 mm 19724202 351.00 USD Add to cart
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13 Tube Rack 175 × 12 mm, bowl, tube support, holder and guide 19868403 413.00 USD Add to cart
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13 Tube Holder and Guide, 95 × 10–18 mm 19868902 230.00 USD Add to cart
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13 Fraction Collector F9-R 29011362 3 538.00 USD Add to cart
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Accessories

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# Product Name Product Code Price
1 Adapter for air sensor 28956342 143.42 USD Add to cart
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2 Bottle holder 28956327 256.00 USD Add to cart
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3 Column Clamp 28956319 70.09 USD Add to cart
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4 Column Holder 28956282 116.24 USD Add to cart
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5 Column Holder Rod 28956270 851.00 USD Add to cart
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6 Flexible column holder 28956295 1 238.00 USD Add to cart
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7 Loop Holder 29011350 761.00 USD Add to cart
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8 Multi-Purpose Holder 29011349 66.86 USD Add to cart
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9 Rail Extension 29011352 371.00 USD Add to cart
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10 Tube holder 28954329 44.13 USD Add to cart
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11 Tubing holder, comb 28956286 69.96 USD Add to cart
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12 Tubing holder, spool, for small tubing 28956274 69.96 USD Add to cart
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Front view

Part Description
1 Fractionation indicator
Symbol indicating that fractionation is ongoing. Do not open the door while the indicator is lit.
2 Door
3 Window
4 Door handle
5 Tubing connector for outlet valve tubing
6 Vents

 

Rear view

Part Description
1 Vents
2 UniNet-9 D-type connector (for communication and power supply)
3 Waste tube

 

Interior

Part Description
1 Fractionation arm guide rail
2 Fractionation arm main rail
3 Lamp
4 Tubing guide
5 Tubing connection
6 Dispenser head
7 Fractionation arm
8 Tubing guide
9 Height exclusion bar
10 Waste funnel
11 Waste tube
12 Tray catch
13 Waste groove, in case of overflow
14 Tray guides

 

Dispenser head

Part Description
1 Dispenser head
2 Nozzle
3 Dispenser head cover
4 Accumulator (back part of Dispenser head)
5 Drop sync sensor
6 Type code reader

 

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# Product Name Product Code Price
UNIPLATE Collection and Analysis Microplate, 24-well, 10 ml, natural polypropylene, round well bottom 28420867 280.00 USD Add to cart
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UNIPLATE Collection and Analysis Microplate, 96-well, 2 ml, natural polypropylene, round well bottom 28420869 222.00 USD Add to cart
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UNIPLATE Collection and Analysis Microplate, 48-well, 5 ml, natural polypropylene, rectangular well, flat bottom 28420874 286.00 USD Add to cart
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Cassette tray 28954209 260.00 USD Add to cart
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Cassette for fraction collection in deep-well plate 28954212 162.51 USD Add to cart
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Cassette for 50 ml tubes 28956402 165.74 USD Add to cart
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Cassette, for 15 ml tubes 28956404 165.74 USD Add to cart
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Cassette for fraction collection in 8 mL tubes 28956425 166.07 USD Add to cart
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Cassette, for 3 ml tubes 28956427 166.07 USD Add to cart
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Rack, for 50 ml tubes 28980319 647.00 USD Add to cart
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Rack for fraction collection in 250 mL bottles 28981873 1 389.00 USD Add to cart
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Fraction Collector F9-C 29027743 10 500.00 USD Add to cart
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Cable 2.5m UniNet-9 D-type 29032425 117.54 USD Add to cart
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# Product Name Product Code Price
Superloop Complete, 150 ml, M6 fitting 18102385 1 358.00 USD Add to cart
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Injection Kit, INV-907 18111089 527.00 USD Add to cart
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Superloop, 1/16" fittings (ÄKTAdesign), 50 ml 18111382 1 139.00 USD Add to cart
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Sample loop 100 µl 18111398 157.13 USD Add to cart
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Sample loop 500 µl 18111399 204.00 USD Add to cart
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Sample loop 1 ml 18111401 171.12 USD Add to cart
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Sample Loop 2.0 ml, INV-907 18111402 255.00 USD Add to cart
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Sample loop, 10 µl 18112039 67.94 USD Add to cart
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Fill Port, INV-907 18112766 223.00 USD Add to cart
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Sample loop, FEP 10 ml 18116124 174.29 USD Add to cart
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Superloop, M6 fitting, 10 ml 19758501 1 564.00 USD Add to cart
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Connector 1/16" Male/Luer Female 28985812 834.90 USD Add to cart
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Up to four external air sensors can be added to ÄKTA pure, and there are two different versions to choose from. They differ in internal diameter and optimal position on the instrument.

The air sensors can be attached to the instrument using the rails and holders. No dummy modules need to be removed.
In addition to be used for preventing air from entering the system, the external air sensors can be used together with System pump A to load the entire sample volume.


Air sensor L9-1.5
L9-1.5 has a 1.5 mm inner diameter and is designed for i.d. 1.6 mm FEP tubing at the low pressure side before pumps. It is installed in the flow path before the system pumps and is used to prevent air entering the valve.


Air sensor L9-1.2
L9-1.2 has a 1.2 mm inner diameter and is designed for o.d. 1/16" tubing at the high pressure side after the pumps. It is installed after the injection valve and is used to prevent air entering the column.

 

Tubing connections 

Air sensor L9-1.5

Connection between Tubing Connector
L9-1.5 and inlet valves FEP, o.d. 1/8", 1.6 mm Tubing connector, 5/16" + Ferrule (yellow), 1/8"

Note: When the air sensor is installed before System pump A, use tubing with a minimum length of 18 cm between the valve located before System pump A and the external air sensor when loading the sample at maximum flow rate, to be sure that air does not reach System pump A. Shorter tubing can be used at lower flow rates.

Air sensor L9-1.2

Connection between Tubing Connector
V9-Inj and L9-1.2 PEEK, o.d. 1/16" Fingertight connector 1/16"
L9-1.2 and V9-C/V9-Cs/the connected column PEEK, o.d. 1/16" Fingertight connector 1/16"

 

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# Product Name Product Code Price
1 Air Sensor L9-1.2 mm 28956502 2 001.00 USD Add to cart
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2 Air Sensor L9-1.5 mm 28956500 2 158.00 USD Add to cart
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Illustration of Fraction collector F9-R, connector panel, base unit and delivery arm.

Part Function
1 Lock knob
2 Delivery arm
3 Tubing connector
4 Tube sensor
5 Collection tubes
6 Tube rack
7 Base unit
8 Node switch
9 UniNet-9 communication and power supply
10 Central spindle
11 Drive sleeve
12 Delivery arm
13 Delivery arm
14 Tube adjustment cavity
15 Sensor control
16 Tubing holder
17 Tubing holder nut
18 Exposed tubing end length

 

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# Product Name Product Code Price
Tube Rack 40 × 30 mm, bowl, tube support, holder and guide 18112467 509.00 USD Add to cart
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Tube Holder and Guide, 40 × 30 mm 18112468 423.00 USD Add to cart
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Tube Rack Complete, 95 × 10–18 mm 18305003 570.00 USD Add to cart
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Bowl 18305103 166.52 USD Add to cart
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Tube Support 18305402 129.15 USD Add to cart
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Tubing Holder 18646401 83.03 USD Add to cart
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Drive sleeve 19606702 108.13 USD Add to cart
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Tube Holder and Guide, 175 × 12 mm 19724202 351.00 USD Add to cart
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Tube Rack 175 × 12 mm, bowl, tube support, holder and guide 19868403 413.00 USD Add to cart
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Tube Holder and Guide, 95 × 10–18 mm 19868902 230.00 USD Add to cart
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Fraction Collector F9-R 29011362 3 538.00 USD Add to cart
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The illustration below shows the location, together with a detailed view of Mixer M9.

Part Description
1 Outlet
2 Mixer chamber
3 Inlet

 

Select Mixer chamber
To obtain a homogeneous buffer composition, it is important to use a Mixer chamber suitable for the flow rate of the method. The tables below show what Mixer chambers to use in ÄKTA pure at different flow rates.
If the liquids are difficult to mix, use a larger Mixer chamber to achieve optimal mixing. However, note that a larger Mixer chamber distorts and delays the gradient.
Three different Mixer chambers are available for ÄKTA pure 25. Their volumes are: 0.6 ml, 1.4 ml (mounted at delivery), and 5 ml.

Mixer chamber volume [ml] Flow rate [ml/min],
Binary gradient
0.6 0.1-5
1.4 0.5-10
5 5-25

 

CAUTION
Risk of explosion
. Do not use Mixer chamber 15 ml with an ÄKTA pure 25 configuration.
The maximum pressure for Mixer chamber 15 ml is 5 MPa.

 

Four different Mixer chambers are available for ÄKTA pure 150. Their volumes are: 0.6 ml, 1.4 ml
(mounted at delivery), 5 ml (included in delivery), and 15 ml.

 

Mixer chamber volume [ml] Flow rate [ml/min],
Binary gradient
0.6 0.5-5
1.4 0.5-15
5 2-50
15 15-150

Note: At low flow rates (below 0.2 ml/min) a gradient of sufficient quality may be achieved with the mixer bypassed.
Note: For the highest flow rates, use the 5 ml mixer for ÄKTA pure 25 and the 15 ml mixer for ÄKTA pure 150. The larger chamber volume minimizes potential pump disturbances.
Note: The 1.4 ml mixer might work up to 25 ml/min provided that the buffers are easily mixed.

Note: O-ring 13.1 x 1.6 mm 28-9535-45 is for use with Mixer chamber 0.6, 1.4 and 5 ml.

Note: O-ring 22.1 x 1.6 mm 28-9818-57 is for use with Mixer chamber 15 ml.

Note: O-ring 13.1 x 1.6 mm 29-0113-26 (high resistant) can be used as an alternative to 29-9535-45.

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# Product Name Product Code Price
Online filter kit 18102711 120.78 USD Add to cart
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O-ring 13.1x1.6 mm 28953545 70.52 USD Add to cart
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Mixer chamber 0.6 ml 28956186 1 134.00 USD Add to cart
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Mixer chamber 1.4 ml 28956225 1 146.00 USD Add to cart
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Mixer chamber 5 ml 28956246 1 146.00 USD Add to cart
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Mixer chamber 15 ml 28980309 1 646.00 USD Add to cart
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O-ring 22.1 x 1.6 mm 28981857 116.46 USD Add to cart
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O-ring 13.1 x 1.6 mm High resistance 29011326 171.12 USD Add to cart
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ÄKTA pure protein purification system: Sample automation

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# Product Name Product Code Price
Sample tubing kit for 7 inlets, i.d. 0.75 mm 28957217 229.00 USD Add to cart
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Mixer Valve Kit V9-M 29011354 2 857.00 USD Add to cart
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Sample Pump S9 29027745 7 309.00 USD Add to cart
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Sample Inlet Valve Kit V9-IS 29027746 5 356.00 USD Add to cart
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Cable 2.5m UniNet-9 D-type 29032425 117.54 USD Add to cart
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Tubing Kit i.d. 1.0 mm 29032426 976.00 USD Add to cart
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Tubing kit for Sample Inlet Valve V9-IS 29035331 258.00 USD Add to cart
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Location and illustration of Conductivity monitor C9n
The illustration below shows the recommended location of Conductivity monitor C9n, together with a detailed view of the monitor. The monitor is labelled C9.

 

Part Description
1 Inlet
2 Conductivity flow cell
3 Conductivity monitor C9n
4 Outlet

 

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# Product Name Product Code Price
Conductivity monitor (C9n) 29011363 2 434.00 USD Add to cart
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Location and illustration of UV monitor U9-M and UV monitor U9-L

The illustration below shows the location of UV monitor U9-M, together with a detailed view of the monitor unit and detector. The monitor unit is labelled U9-M and the detector U9-D.

Note: When UV monitor U9-M is used, the entire Multi-module panel shown in the illustration is replaced by U9-M.

The illustration below also shows the recommended location of UV monitor U9-L, together with a detailed view of the monitor and detector. The UV monitor U9-L requires that the Multi-module panel is installed.

Part Description
1 Inlet
2 UV flow cell. Three different path lengths are available: 0.5 mm, 2 mm (default) and 10 mm
3 UV detector
4 Outlet
5 Inlet
6 UV monitor U9-L
7 UV flow cell. Two different path lengths are available: 2 mm (default) and 5 mm
8 Outlet
9 UV detector

 

Function of UV monitor U9-L

The UV monitor U9-L measures the UV absorbance at the fixed wavelength of 280 nm. It is not possible to vary the wavelength, or turn on or off the U9-L monitor. This is therefore not shown in the Phase Properties pane in Method Editor.

For information on how to install UV monitor U9-L, please refer to Section 4.10 Install UV monitor U9-L, in the system handbook.

 

Using two UV monitors
The UV monitor U9-L module can be used in two configurations, UV monitor U9-L and UV monitor U9-L, 2nd. The configuration is defined by the module's Node ID.
It is possible to use two UV monitors in ÄKTA pure, in the following combinations:
• UV monitor U9-M together with UV monitor U9-L, 2nd

• UV monitor U9-L together with UV monitor U9-L, 2nd

UV monitor U9-L, 2nd can be located anywhere in the flow path and is therefore shown in the Process Picture as a component without a fixed place. This means that it is possible to place U9-L, 2nd before the other UV monitor in the flow path.
Note: If U9-L, 2nd is placed on the high pressure side of the column, pressure limits have to be considered.

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# Product Name Product Code Price
UV flow cell, 5 mm, for U9-L 18112824 2 166.00 USD Add to cart
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UV flow cell, 10 mm, for U9-M 28956378 1 987.00 USD Add to cart
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UV flow cell, 2 mm, for U9-M 28979380 2 954.00 USD Add to cart
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UV flow cell, 0.5 mm, for U9-M 28979386 3 067.00 USD Add to cart
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UV flow cell, 2 mm, for U9-L 29011325 2 372.00 USD Add to cart
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UV Monitor U9-L 29011360 4 994.00 USD Add to cart
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The modular design allows the user to customize ÄKTA pure in multiple ways. The system is always delivered with the core modules of the selected configuration, but one or more optional modules may be added to the flow path.
The table below contains information on the core modules and the optional modules of ÄKTA pure 25 and ÄKTA pure 150. 

The valves for ÄKTA pure 25 and ÄKTA pure 150 are compatible with both systems but for the best performance the specific valve type should be used.

Note: All valve kits include the necessary tubing.

Module Label in ÄKTA pure 25 Label in ÄKTA pure 150
System pump A P9 A P9H A
System pump B P9 B P9H B
Pressure monitor R9 R9
Mixer M9 M9
Injection valve V9-Inj V9H-Inj
Inlet valve A V9-IA V9H-IA
Inlet valve B V9-IB V9H-IB
Inlet valve AB V9-IAB V9H-IAB
Inlet valve IX V9-IX V9H-IX
Sample inlet valve V9-IS V9H-IS
Mixer valve V9-M V9H-M
Loop valve V9-L V9H-L
Column valves

V9-C

V9-Cs

V9H-C

V9H-Cs

pH valve V9-pH V9H-pH
Outlet valves

V9-O

V9-Os

V9H-O

V9H-Os

Versatile valve V9-V V9H-V
UV monitors

U9-L

U9-M

U9-L

U9-M

Conductivity monitor C9 C9
External air sensor

L9-1.5

L9-1.2

L9-1.5

L9-1.2

Fraction collectors

F9-C

F9-R

F9-C

F9-R

I/O box E9 E9
Sample pump S9 S9H

 

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# Product Name Product Code Price
Injection Valve V9-Inj 28956514 3 404.00 USD Add to cart
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Inlet Valve V9-X1 28957227 3 402.00 USD Add to cart
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Inlet Valve V9-X2 28957234 3 409.00 USD Add to cart
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Inlet Valve V9H-X1 28979326 3 359.00 USD Add to cart
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Inlet Valve V9H-X2 28979328 3 359.00 USD Add to cart
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Versatile valve V9-V 29011353 2 622.00 USD Add to cart
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Mixer Valve Kit V9-M 29011354 2 857.00 USD Add to cart
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Column Valve V9-Cs 29011355 3 111.00 USD Add to cart
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Outlet Valve Kit V9-Os 29011356 2 117.00 USD Add to cart
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Inlet Valve Kit V9-IAB 29011357 2 737.00 USD Add to cart
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Loop valve V9-L 29011358 5 129.00 USD Add to cart
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pH Valve Kit V9-pH 29011359 2 732.00 USD Add to cart
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Column Valve kit V9-C 29011367 7 342.00 USD Add to cart
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Outlet valve kit V9-O 29012261 3 610.00 USD Add to cart
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Inlet Valve Kit V9-IA 29012263 5 477.00 USD Add to cart
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Inlet Valve Kit V9-IB 29012370 5 477.00 USD Add to cart
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Sample Inlet Valve Kit V9-IS 29027746 5 356.00 USD Add to cart
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Sample Inlet Valve Kit V9H-IS 29050943 5 466.00 USD Add to cart
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Inlet Valve Kit V9H-IA 29050945 5 477.00 USD Add to cart
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Inlet Valve Kit V9H-IB 29050946 5 477.00 USD Add to cart
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Outlet Valve Kit V9H-O 29050949 3 610.00 USD Add to cart
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Column Valve Kit V9H-C 29050951 7 342.00 USD Add to cart
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pH Valve Kit V9H-pH 29051684 2 737.00 USD Add to cart
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Inlet Valve Kit V9H-IAB 29089652 2 684.00 USD Add to cart
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Loop valve kit V9H-L 29090689 5 129.00 USD Add to cart
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Versatile valve V9H-V 29090691 2 569.00 USD Add to cart
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Mixer Valve Kit V9H-M 29090692 2 806.00 USD Add to cart
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Column Valve V9H-Cs 29090693 3 050.00 USD Add to cart
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Outlet Valve Kit V9H-Os 29090694 2 080.00 USD Add to cart
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Location and illustration of the pH monitor
The illustration below shows the location of the pH flow cell and a pH electrode installed in the pH valve.

Part Description
1 pH flow cell
2 pH electrode

 

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# Product Name Product Code Price
1 O-ring 5.3 x 2.4 mm 28956497 133.46 USD Add to cart
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2 pH electrode 29387193 661.00 USD Add to cart
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Flow path schemes

The illustration below shows the flow path for a typical system configuration. The individual instrument modules are presented in the table below. The configuration of the system is defined by the user.

Part Description
1 Inlet valve V9-IAB
2 System pump B
3 System pump A
4 Pressure monitor R9
5 Mixer M9
6 Injection valve V9-Inj
7 Capillary loop or Superloop
8 Column valve V9-Cs
9 Column
10 UV monitor U9-L
11 Conductivity monitor C9n
12 Flow restrictor
13 Outlet valve V9-Os
14 Fraction collector F9-R
W, W1, W2 Waste

 

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Tubing types

The table below shows the tubing types used in ÄKTA pure.

Description Color Scope of use
PEEK, o.d. 1/16", i.d. 0.25 mm Blue Reference capillary 1 and Tubing Kit 0.25
PEEK, o.d. 1/16", i.d. 0.50 mm Orange High pressure tubing
PEEK, o.d. 1/16", i.d. 0.75 mm Green High pressure tubing
FEP, o.d. 1/8", i.d. 1.6 mm Transparent Inlet tubing
ETFE, o.d. 1/16", i.d. 1.0 mm Transparent Outlet and waste tubing
Silicon, o.d. 12 mm, i.d. 8 mm Transparent Waste tubing from Buffer tray

Note: Many different sizes/types of tubing can be connected to a chromatography system. Tubing with a smaller inner diameter (i.d.) holds less delay volume and will therefore generate less dilution of the protein peak. Narrow tubing, however, increases the system pressure, especially when running at high flow rates. The tubing used should match the application needs. See Recommended tubing kits, on page 467 in the System Handbook for more information.

 

Tubing connectors
The table below shows the tubing connectors used in ÄKTA pure.

Description Use with tubing...
Fingertight connector, 1/16" • PEEK, o.d. 1/16", i.d. 0.25 mm
• PEEK, o.d. 1/16", i.d. 0.50 mm
• PEEK, o.d. 1/16", i.d. 0.75 mm
• ETFE, o.d. 1/16", i.d. 1 mm
Tubing connector 5/16"
Ferrule (yellow), 1/8"
FEP, o.d. 1/8", i.d. 1.6 mm

 

Other connectors
The table below shows other connectors used in ÄKTA pure.

Description Scope of use
Stop plug 1/16" Stop plug for valve parts
Luer female Syringe connector for pH valve and Injection valve

 

Tubing labels
The illustration below shows the tubing labels for for a typical system configuration.

 

Inlet tubing
The table below shows the labels, diameters, and standard lengths of the inlet tubing.

Label Description Tubing Length (mm)
A1-A2  
and
B1-B2
 Inlets to Inlet valve AB FEP, o.d. 1/8", i.d. 1.6 mm 1500
A1-A7 Inlets to Inlet valve A FEP, o.d. 1/8", i.d. 1.6 mm 1500
B1-B7 Inlets to Inlet valve B FEP, o.d. 1/8", i.d. 1.6 mm 1500
InA FromInlet valve A or Inlet valve AB to System pump A FEP, o.d. 1/8", i.d. 1.6 mm 220
InB FromInlet valve B or Inlet valve AB to System pump B FEP, o.d. 1/8", i.d. 1.6 mm 220

 

High pressure tubing
The table below shows the labels, diameters, and standard lengths of the high pressure tubing.

Label Description Tubing Length
(mm)
1A1 System pump A left to Restrictor A PEEK, o.d. 1/16", i.d. 0.75 mm 340
1A2 System pump A right to Restrictor A PEEK, o.d. 1/16", i.d. 0.75 mm 340
2A Restrictor A to Pressure monitor PEEK, o.d. 1/16", i.d. 0.75 mm 115
1B1 System pump B left to Restrictor B PEEK, o.d. 1/16", i.d. 0.75 mm 340
1B2 System pump B right to Restrictor B PEEK, o.d. 1/16", i.d. 0.75 mm 340
2B Restrictor B to Pressure monitor PEEK, o.d. 1/16", i.d. 0.75 mm 115
3 Pressure monitor to Mixer PEEK, o.d. 1/16", i.d. 0.75 mm 400
4 Mixer to Injection valve PEEK, o.d. 1/16", i.d. 0.75 mm 200
5 Injection valve to Column valve PEEK, o.d. 1/16", i.d. 0.75 mm 160
6 Column valve to UV monitor PEEK, o.d. 1/16", i.d. 0.75 mm 160
7 UV monitor to Conductivy monitor PEEK, o.d. 1/16", i.d. 0.75 mm 170
8 Conductivity monitor to Flow restrictor PEEK, o.d. 1/16", i.d. 0.75 mm 95
9 Flow restrictor to Outlet valve PEEK, o.d. 1/16", i.d. 0.75 mm 135
Frac Outlet valve to Fraction collector PEEK, o.d. 1/16", i.d. 0.75 mm 400

 

Reference capillaries
The table below shows the labels, diameters, and standard lengths of the reference capillary. The capillary is used during the System performance tests.

Label Description Tubing Length (mm)
Ref 1 Reference capillary PEEK, o.d. 1/16", i.d. 0.25 mm 400

 

Outlet tubing
The table below shows the labels, diameters, and standard lengths of the outlet tubing.
The tubing is not mounted on delivery.

Label Description Tubing Length (mm)
Out1-Out 10 Outlets from Outlet valve V9-O and Outlet valve V9-Os ETFE, o.d. 1/8", i.d. 1 mm 1500

 

Waste tubing
The table below shows the labels, diameters, and standard lengths of the waste tubing. The waste tubing is mounted on delivery.

Label Description Tubing Length (mm)
W1 System pump waste ETFE, o.d. 1/16", i.d. 1.0 mm 1500
W2 Sample pump waste ETFE, o.d. 1/16", i.d. 1.0 mm 1500
W3 pH valve waste ETFE, o.d. 1/16", i.d. 1.0 mm 1500
W System waste ETFE, o.d. 1/16", i.d. 1.0 mm 1500
N/A Top tray waste Silicon, o.d. 12 mm, i.d. 8 mm 1500

 

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Troubleshooting

Find solutions to product related issues. For unlisted issues please contact local Cytiva service representation.

Mixer valve

Issues related to the Mixer valve

Outlet valve

Issues related to the Outlet valve

Instrument communication

Issues related to Instrument communication

Cabinet

Issues related to the cabinet

General hardware: All modules

Issues related to general hardware: All modules

Superloop

Issues related to the Superloop

I/O-box E9

Issues related to I/O-box E9

Pumps

Issues related to the Pumps

Instrument control panel

Issues related to the Instrument control panel

Versatile valve

Issues related to the Versatile valve

Examples of pump pressure curves

The table below shows some examples of pump system pressure curves obtained when errors have occurred. The examples can be useful in troubleshooting of the System pumps. The System pressure monitor R9 has higher resolution than the other pressure monitors, and is therefore recommended for troubleshooting purposes.

 

Incident: 30 μl of air enters pump.
Action: Purge pump. See Section 5.4 Prime inlets and purge pump heads, on page 217 in the system handbook.

Incident: A large volume of air enters pump.
Action: Purge pump. See Section 5.4 Prime inlets and purge pump heads, on page 217.

Incident: Blocked outlet check valve
Action: Clean the check valve, See Section 7.6.7 Clean the pump head check valves, on page 343 in the system handbook.

Incident: Inlet check valve is loose.
Action: Tighten the check valve. See Section 7.8.8 Replace the pump head check valves, on page 378 in the system handbook.

Incident: Leaking inlet check valve.
Action: Replace the check valve. See Section 7.8.8 Replace the pump head check valves, on page 378 in the system handbook.

Incident: Leaking outlet check valve.
Actions: Replace the check valve. See Section 7.8.8 Replace the pump head check valves, on page 378 in the system handbook.

Incident: One inlet is blocked,
Action: Clean inlet tubing. For example, perform a System CIP.

Loop valve

Issues related to Loop valve

Inlet valves

Issues related to Inlet valves. 

The Inlet valves include Inlet valve A, Inlet valve B and Inlet valve AB.

Fraction collector F9-R

Issues related to Fraction collector F9-R

Injection valve

Issues related to Injection valve

pH monitor and pH valve

Issues related to pH monitor and pH valve

Dummy modules

Issues related to Dummy modules

Column valve

Issues related to Column valve

Fraction collector F9-C

Issues related to Fraction collector F9-C

Method development

Issues related to Method development

General advice to achieve good performance

Before using the system make sure that:

  • The correct system has been selected in UNICORN System Control
  • The correct wavelength has been set for UV/UPC monitor
  • All tubing has been properly connected
  • All connectors are free from leakage
  • No tubing is folded or twisted
  • Online filter, if used, is changed on a regular basis
  • Correct buffers are used for the chosen columns and proteins
  • All inlet tubing has been immersed in correct buffer solutions
  • Enough buffer has been prepared
  • Buffers have been equilibrated to the environment temperature
  • Buffers/eluents have been degassed if necessary (e.g., in RPC runs)
  • Suitable columns have been selected for the target proteins
  • Columns have been cleaned and prepared according to column instructions
  • Samples have been clarified by centrifugation and/or filtration prior to sample loading
  • Samples have been adjusted to binding buffer conditions
  • Auto sampler (if used) has been prepared according to user manual
  • The fraction collector has been filled with appropriate number of microtiter plates or tubes
  • Appropriate arrangement for waste handling has been prepared

UV monitor U9-L

Issues related to UV monitor U9-L

Pressure monitors

Issues related to Pressure monitors

Power and ICU

Issues related to Power and ICU

Conductivity monitor

Issues related to the Conductivity monitor

UV monitor U9-M and UV detector unit

Issues related to UV monitor and UV detector unit

Error codes

All modules

Error code Description Action
0 - 19 Internal instrument error Restart the instrument. If recurrent please contact Service.

 

Instrument control unit

Error code Description Action
21 - 69 Internal instrument error Restart the instrument. If recurrent please contact Service.

 

Valve

Error code Description Action
20, 22, 24 Internal instrument error Restart the instrument. If recurrent please contact Service.
23 Faulty air sensor Restart the instrument. If recurrent please contact Service.
25 High temperature See Section 8.3 Troubleshooting: Valves on page 414 in the system handbook.

 

Pressure monitor

Error code Description Action
20, 21, 24-27 Internal instrument error Restart the instrument. If recurrent please contact Service.
23 High temperature See Pressure monitors, on page 411 in the system handbook.

 

Air sensor

Error code Description Action
20 High temperature See External air sensors, on page 435 in the system handbook.

 

Mixer

Error code Description Action
25 Mixer motor error Restart the instrument. If recurrent please contact Service.
26 Internal instrument error Restart the instrument. If recurrent please contact Service.
27 High temperature See Mixer, on page 431 in the system handbook.

 

pH monitor

Error code Description Action
20, 21 Internal instrument error Restart the instrument. If recurrent please contact Service.
25 No factory calibration Contact Service
26 High temperature See pH monitor and pH valve, on page409 in the system handbook.

 

Conductivity monitor

Error code Description Action
20-27 Internal instrument error Restart the instrument. If recurrent please contact Service.
28 High temperature See Conductivity monitor, on page 405 in the system handbook.
29 Temperature data error Restart the instrument. If recurrent please contact Service.
32-34 No factory calibration Contact Service

 

Pump

Error code Description Action
51-53 Mixer motor error Restart the instrument. If recurrent please contact Service.
54 Internal instrument error See Section 8.5 Troubleshooting: Pumps, on page 424 in the system handbook.

 

UV monitor U9-M

Error code Description Action
21, 25, 26, 31 Grating error Restart the instrument. If recurrent please contact Service.
22, 23, 32 Block filter error Restart the instrument. If recurrent please contact Service.
24 Internal instrument error Restart the instrument. If recurrent please contact Service.
27 Spectrum calibration error Restart the instrument. If recurrent please contact Service.
28, 29 Lamp error Restart the instrument. If recurrent please contact Service.
30 High temperature See UV monitor U9-M and UV detector unit, on page 398 in the system handbook.

 

UV detector U9-D

Error code Description Action
24, 26, 28, 29, 31, 33 Internal instrument error Restart the instrument. If recurrent please contact Service.
25 Too low light intensity Check that the detector and flow cell are fitted correctly. If warning reappears, contact Service.
27 Too high light intensity Check that the detector and flow cell are fitted correctly. If warning reappears, contact Service.
30 Too high light intensity, R channel. Check that the detector is fitted correctly. If warning reappears, contact Service.
32 Too high S light intensity, S channel. Check that the flow cell is fitted correctly. If warning reappears, contact Service.
34 No light detected Check optical pathway and restart the instrument. If recurrent please contact Service.
35 Too low light intensity, R channel. Check that the detector is fitted correctly. If warning reappears, contact Service.
36 Too low light intensity, S channel. No light through flow cell. Check solution absorption and that the cell is fitted correctly.

 

UV monitor U9-L

Error code Description Action
51 High temperature See UV monitor U9-L, on page 403 in the system handbook.
52, 55 Low lamp intensity Contact Service
54 Autozero out of range Autozero requested when AU value is larger than 2.
58 Too low light intensity, S channel. No light through flow cell. Check solution absorption and that the cell is fitted correctly.
59, 60 Internal instrument error Restart the instrument. If recurrent please contact Service.
61 Measurement error Restart the instrument. If recurrent please contact Service.

 

Fraction collector F9-R

Error code Description Action
20 High temperature See Section 8.4 Troubleshooting: Fraction collector, on page 422 in the system handbook.
21 Drop sync warning Clean the sensor and remove air bubbles in the flow path.
22 Tube sensor error Check that the tube sensor is adjusted properly.
23 Delay queue full Increase the fraction size. Fraction size must be greater than 1/10 of the delay volume.
24, 26 Internal instrument error Restart the instrument. If recurrent please contact Service.
25, 28 Too fast tube change Increase the fraction size or lower the flow rate.
27 Drop sync error Clean the drop sensor

 

Instrument control panel

Error code Description Action
51 High temperature See Instrument control panel, on page 437 in the system handbook.

 

I/O-box

Error code Description Action
20 High temperature See section "Troubleshooting"
21 Analog in signal below -2V Check the external equipment connected to the I/O-box.
22 Analog in signal above 2V Check the external equipment connected to the I/O-box.
23-28 Internal instrument error Restart the instrument. If recurrent please contact Service.

 

External air sensors

Issues related to External air sensors

pH valve

Issues related to pH valve

Maintenance

Maintenance instructions and procedures.

ÄKTA pure - periodic maintenance program

This section lists the periodic maintenance activities that should be performed by the ÄKTA pure users, as well as maintenance activities that should be performed when required.

Warning

Hazardous biological agents during run. When using hazardous biological agents, run System CIP and Column CIP to flush the entire system tubing with bacteriostatic solution (e.g., NaOH) followed by a neutral buffer and finally distilled water, before service and maintenance.

Caution

Hazardous chemicals during run. When using hazardous chemicals, run System CIP and Column CIP to flush the entire system tubing with distilled water, before service and maintenance.

Caution

Always use appropriate personal protective equipment during operation and maintenance.

Maintenance operations should be performed by the user at regular intervals

Calibrate the pH monitor

The detailed procedures can be found in the ÄKTA pure System Handbook located in the related documents tab.

Maintenance See section in ÄKTA pure System Handbook
Calibrate the pH monitor See section 7.7.1 Calibrate the pH monitor.

 

Calibrate pressure monitors

The following periodic maintenance should be performed by the user of ÄKTA pure weekly.

Maintenance See section in ÄKTA pure System handbook
Calibrate pressure monitors Section 7.7.2 Calibrate the pressure monitors

 

Change pump rinsing solution

The detailed procedures can be found in the ÄKTA pure System Handbook located in the related documents tab.

Maintenance See section in ÄKTA pure System handbook
Change pump rinsing solution Section 7.3.1 Change pump rinsing solution

 

Check the Flow restrictor

The following periodic maintenance should be performed by the user of ÄKTA pure, monthly.

Maintenance See section in the ÄKTA pure System Handbook
Check the Flow restrictor Section 7.4.1 Check the Flow restrictor

 

Clean the UV flow cell

The following periodic maintenance should be performed by the user of ÄKTA pure, bi-annually.

Maintenance See section in the ÄKTA pure System Handbook
Clean the UV flow cell Section 7.5.1 Clean the UV flow cell

 

Replace pH electrode

The following periodic maintenance should be performed by the user of ÄKTA pure, bi-annually.

Maintenance See section in the ÄKTA pure System Handbook
Replace pH electrode Section 7.5.2 Replace the pH electrode

 

The following maintenance should be performed by the user of ÄKTA pure when required
Maintenance action See section in the ÄKTA pure System Handbook
Clean the instrument externally Section 7.6.1 Clean the instrument externally
Perform System CIP Section 7.6.2 Perform System CIP
Perform Column CIP Section 7.6.3 Perform Column CIP
Clean the Fraction collector F9-R Section 7.6.4 Clean the Fraction collector
Replace tubing and connectors Section 7.8.1 Replace tubing and connectors
Storage of pH electrode Section 7.6.5 Storage of the pH electrode
Clean the pH electrode Section 7.6.6 Clean the pH electrode
Clean the Conductivity flow cell Section 7.6.8 Clean the Conductivity flow cell
Calibrate the Conductivity monitor Section 7.7.3 Calibrate the Conductivity monitor
Calibrate the UV monitor Section 7.7.4 Calibrate the UV monitors
Replace Mixer Section 7.8.2 Replace the Mixer
Replace inline filter Section7.3.2 Replace the inline filter
Replace O-ring in Mixer Section 7.8.3 Replace the O-ring of the Mixer
Replace UV flow cell Section 7.8.4 Replace the UV monitor U9-M flow
cell
Replace the Flow restrictor Section 7.8.6 Replace the Flow restrictor
Replace inlet filters Section 7.8.7 Replace the inlet filters
Clean the check valves Section 7.6.7 Clean the pump head check valves
Replace check valves Section 7.8.8 Replace the pump head check
valves
Replace pump piston seals Section 7.8.9 Replace pump piston seals of Pump
P9
Replace pump pistons Section 7.8.10 Replace pump pistons
Replace pump rinsing system
tubing
Section 7.8.11 Replace pump rinsing system
tubing

 

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