FAQ

Spare parts

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# Product Name Product Code Price
1 Sealing Kit 18110212 136.86 USD Add to cart
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2 O-Ring, FineLINE Pilot 35, 28.3 × 2.6 mm, FFPM/FFKM 18110215 695.00 USD Add to cart
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# Product Name Product Code Price
1 Adaptor knob 18114833 466.00 USD Add to cart
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2 Adaptor lid 18114764 372.00 USD Add to cart
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5 TUBING FLANG 1,2X420 FINELINE 18110220 61.37 USD Add to cart
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6 Adaptor tube including screws 18114765 748.00 USD Add to cart
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8 Domed nuts M6 incl washer 18114766 112.67 USD Add to cart
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13 Glass tube 18110216 529.00 USD Add to cart
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15 Adaptor filter unit 2 µm 18110210 570.00 USD Add to cart
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18 Bottom filter unit 2 µm 18110211 536.00 USD Add to cart
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18 Bottom end-piece 18114768 522.00 USD Add to cart
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19 Nuts M6 incl washer 18114769 274.00 USD Add to cart
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20 Sealing Kit 18110212 136.86 USD Add to cart
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20 Check valve assembly 18114770 318.00 USD Add to cart
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21 Check valve 18110213 97.28 USD Add to cart
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Sealing Kit 18110212 136.86 USD Add to cart
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O-Ring, FineLINE Pilot 35, 28.3 × 2.6 mm, FFPM/FFKM 18110215 695.00 USD Add to cart
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Accessories

Make sure you have all connectors and tubing needed for running the column.

Depending on your system configuration and column length you may need extra long tubing to connect your column.

Please do not extend the tubing unnecessarily which results in a lower resolution.

* Connect M6 male, to 5/16" (18-1169-16) for ÄKTApilot and 1/16" (18-1112-58) for ÄKTA lab systems.

Something went wrong - we were not able to load your agreed MSA pricing. Please try refreshing the page.
# Product Name Product Code Price
1 TUBING FLANG 1,2X420 FINELINE 18110220 61.37 USD Add to cart
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1 Tubing cutter, for PEEK, EFTE, and FEP tubing i.d. 0.25, 0.5, 0.75, 1 and 1.6 mm 18111246 80.74 USD Add to cart
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1 Outlet tubing P6000 18454601 47.18 USD Add to cart
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3 Fingertight Union 1/16" Male/M6 Female 18111258 111.71 USD Add to cart
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3 Union, M6 Female - 5/16" Male 18116916 105.47 USD Add to cart
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Make sure you have all connectors and tubing needed for running the column.

Depending on your system configuration and column length you may need extra long tubing to connect your column. 

Please do not extend the tubing unnecessarily which results in a lower resolution.

Something went wrong - we were not able to load your agreed MSA pricing. Please try refreshing the page.
# Product Name Product Code Price
1 FEP Tubing, i.d. 1/8 in (3/16 in) 18111247 88.60 USD Add to cart
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2 Connector 5/16" Female - M6 Male 18112776 55.00 USD Add to cart
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3 Ferrules for 3/16" o.d. tubing connector 18111248 107.86 USD Add to cart
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4 Connector 3/16" Male for Tubing o.d. 3/16" 18111249 259.00 USD Add to cart
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5 TUBING FLANG 1,2X420 FINELINE 18110220 61.37 USD Add to cart
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5 Tubing cutter, for PEEK, EFTE, and FEP tubing i.d. 0.25, 0.5, 0.75, 1 and 1.6 mm 18111246 80.74 USD Add to cart
Get Quote
5 Outlet tubing P6000 18454601 47.18 USD Add to cart
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6 Tubing connector SRTC-3 19214401 140.19 USD Add to cart
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8 Fingertight Union 1/16" Male/M6 Female 18111258 111.71 USD Add to cart
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11 Pressure Relief Valve for FineLINE Pilot 35 Column 18111090 715.00 USD Add to cart
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13 Signal Cable, (6-pin miniDin-Open) 18111064 321.00 USD Add to cart
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13 Rinsing/draining housing 18111203 90.84 USD Add to cart
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13 Piston kit 100 ml 18111213 606.00 USD Add to cart
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13 Seal kit 100 ml 18111312 775.00 USD Add to cart
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13 Tubing Kit for Rinsing System 18111332 357.00 USD Add to cart
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13 Pumphead capillaries 18111752 108.93 USD Add to cart
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13 New Purge Kit P-900 18112453 76.56 USD Add to cart
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13 Pumphead complete 100 ml 18112848 1 867.00 USD Add to cart
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13 Check valve kit 18112866 1 134.00 USD Add to cart
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13 Purge valve 18112887 205.00 USD Add to cart
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13 O-Ring, 1 × 3 mm, PFR 19003601 73.60 USD Add to cart
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15 pH Electrode, round tip 18111126 1 473.00 USD Add to cart
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Make sure you have all connectors and tubing needed for packing and running the column.

Depending on your system configuration and column length, you may need extra long tubing to connect your column.

Something went wrong - we were not able to load your agreed MSA pricing. Please try refreshing the page.
# Product Name Product Code Price
1 FEP Tubing, i.d. 1/8 in (3/16 in) 18111247 88.60 USD Add to cart
Get Quote
1 Ferrules for 3/16" o.d. tubing connector 18111248 107.86 USD Add to cart
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1 Connector 3/16" Male for Tubing o.d. 3/16" 18111249 259.00 USD Add to cart
Get Quote
2 TUBING FLANG 1,2X420 FINELINE 18110220 61.37 USD Add to cart
Get Quote
2 Tubing cutter, for PEEK, EFTE, and FEP tubing i.d. 0.25, 0.5, 0.75, 1 and 1.6 mm 18111246 80.74 USD Add to cart
Get Quote
2 Outlet tubing P6000 18454601 47.18 USD Add to cart
Get Quote
3 Tubing connector SRTC-3 19214401 140.19 USD Add to cart
Get Quote
5 Fingertight Union 1/16" Male/M6 Female 18111258 111.71 USD Add to cart
Get Quote
6 Valve SRV-1 19214501 242.00 USD Add to cart
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7 Pressure Relief Valve for FineLINE Pilot 35 Column 18111090 715.00 USD Add to cart
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9 Signal Cable, (6-pin miniDin-Open) 18111064 321.00 USD Add to cart
Get Quote
9 Rinsing/draining housing 18111203 90.84 USD Add to cart
Get Quote
9 Piston kit 100 ml 18111213 606.00 USD Add to cart
Get Quote
9 Seal kit 100 ml 18111312 775.00 USD Add to cart
Get Quote
9 Tubing Kit for Rinsing System 18111332 357.00 USD Add to cart
Get Quote
9 Pumphead capillaries 18111752 108.93 USD Add to cart
Get Quote
9 New Purge Kit P-900 18112453 76.56 USD Add to cart
Get Quote
9 Pumphead complete 100 ml 18112848 1 867.00 USD Add to cart
Get Quote
9 Check valve kit 18112866 1 134.00 USD Add to cart
Get Quote
9 Purge valve 18112887 205.00 USD Add to cart
Get Quote
9 O-Ring, 1 × 3 mm, PFR 19003601 73.60 USD Add to cart
Get Quote

Make sure you have all connectors and tubing needed for packing and running the column.

Depending on your system configuration and column length, you may need extra long tubing to connect your column.

* Connect M6 male, to 5/16" (18-1169-16) for ÄKTApilot and 1/16" (18-1112-58) for ÄKTA lab systems.

Something went wrong - we were not able to load your agreed MSA pricing. Please try refreshing the page.
# Product Name Product Code Price
1 TUBING FLANG 1,2X420 FINELINE 18110220 61.37 USD Add to cart
Get Quote
1 Tubing cutter, for PEEK, EFTE, and FEP tubing i.d. 0.25, 0.5, 0.75, 1 and 1.6 mm 18111246 80.74 USD Add to cart
Get Quote
1 Outlet tubing P6000 18454601 47.18 USD Add to cart
Get Quote
3 Valve SRV-1 19214501 242.00 USD Add to cart
Get Quote
5 Fingertight Union 1/16" Male/M6 Female 18111258 111.71 USD Add to cart
Get Quote
5 Union, M6 Female - 5/16" Male 18116916 105.47 USD Add to cart
Get Quote

Troubleshooting

Find solutions to product related issues. For unlisted issues please contact local Cytiva service representation.

General advice to achieve good performance

Before packing or running the column please make sure that

  • the tubing, valves, gaskets etc. have the same inner diameter as the column in and outlets and tubing is not longer than needed.
  • the bed supports have a porosity appropriate for your chromatography medium.
  • the column has been correctly assembled, verified by passing a leakage test.
  • the chromatography medium and equipment are equilibrated in the packing buffer and to ambient temperature of packing environment.
  • that the pump is free from pulsations.
  • no pressure peaks appear.
  • that you have enough slurry volume, taken in account the compression factor of the medium.
  • packing is performed at optimal flow velocity.
  • the column meets pressure requirement for selected medium and packing procedure.

After completing the packing procedure please evaluate the efficiency of the column in terms of the height equivalent to a theoretical plate (HETP), reduced plate number (h) and the peak asymmetry factor (As).


Between batches it is recommended to perform cleaning-in-place (CIP) to remove precipitated material, strongly bound substances and other contaminants from the column bed without dismantling the column. Regular CIP is needed to ensure proper product quality and expected life time of medium and equipment.

Many issues that face chromatographers are common to all types of columns, while other issues are quite specific.
Select the appropriate equipment.

Worrying peak shape

Strange peaks

Column leakage

Poor reproducibility

Back pressure increases during operation

Unusual column appearance

Poor product recovery

Drifting baseline

Poor packing evaluation result

On this troubleshooting site, we refer to leading and tailing peaks as defined according to the following figures.


Leading peak

Tailing peak

 
Experience shows that the best method of expressing the efficiency of a packed column is in terms of its height equivalent to a theoretical plate (HETP), reduced plate number (h) and its peak asymmetry factor (As).

Unsatisfactory elution

Contact