FAQ

Spare parts

Figure. HiScale 50 column.

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# Product Name Product Code Price
1 Adapter complete for HiScale 50 28966385 776.28 USD Add to cart
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7 Column tube assembly 28966649 1 248.56 USD Add to cart
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7 Column tube assembly 28966650 1 872.83 USD Add to cart
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8 Stop Plug 5/16" Male 18111250 33.21 USD Add to cart
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9 Connector, 5/16" Female - 5/16" Female 18117351 221.00 USD Add to cart
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11 Connector for 1/8" tubing 18112117 183.85 USD Add to cart
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16 Capilary tubing 28966656 92.28 USD Add to cart
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19 Ferrule for o.d. 1/8" Tubing 18112118 134.04 USD Add to cart
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22 O-Ring 20.3x 2.62 mm 28978227 70.57 USD Add to cart
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24 Support Screen for Column, i.d. 50 mm 19066401 93.37 USD Add to cart
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25 Net ring 20 µm 28966381 115.08 USD Add to cart
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Accessory kit for HiScale 50 columns 28966375 150.91 USD Add to cart
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O-rings and nets should be checked regularly for wear. Worn O-rings may not seal properly and over-used nets can affect distribution. Inspect the O-rings and evaluate the height equivalent to a theoretical plate (HETP) and peak asymmetry factor (As) to prevent poor performance due to old nets. 

We recommend you to change the nets and O-rings when needed or at least annually. Please keep a set of each on your site, for the code numbers refer to the table below.

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# Product Name Product Code Price
1 Tubing 28966376 124.86 USD Add to cart
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2 Support Screen for Column, i.d. 50 mm 19066401 93.37 USD Add to cart
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3 O-ring Adapter, XK 50 28978228 102.06 USD Add to cart
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4 Net ring 20 µm 28966381 115.08 USD Add to cart
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5 Spanner wrench 28964778 115.08 USD Add to cart
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6 Ferrule for o.d. 1/8" Tubing 18112118 134.04 USD Add to cart
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7 Accessory kit for HiScale 50 columns 28966375 150.91 USD Add to cart
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Accessories

 

Make sure you have all connectors and tubing needed for packing and running the column.

Depending on your system configuration and column length, you may need extra long tubing to connect your column.

2x Connector 1/8", 2x Union 5/16" female - 5/16" female, 2x Stop plug 5/16" and 2x Ferrules 1/8" are supplied with the column.

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# Product Name Product Code Price
1 Tubing 28966376 124.86 USD Add to cart
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2 Connector for 1/8" tubing 18112117 183.85 USD Add to cart
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3 Connector, 5/16" Female - 5/16" Female 18117351 221.00 USD Add to cart
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4 Stop Plug 5/16" Male 18111250 33.21 USD Add to cart
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5 Ferrule for o.d. 1/8" Tubing 18112118 134.04 USD Add to cart
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6 Column tube assembly 28966649 1 248.56 USD Add to cart
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6 Column tube assembly 28966650 1 872.83 USD Add to cart
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7 Union 5/16" female - 1/16" male 18114208 52.20 USD Add to cart
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Tubing cutter, for PEEK, EFTE, and FEP tubing i.d. 0.25, 0.5, 0.75, 1 and 1.6 mm 18111246 80.74 USD Add to cart
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Column holder for HiScale 50 28964499 485.31 USD Add to cart
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Packing tube 40 for HiScale 50 columns 28964506 2 375.51 USD Add to cart
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Spanner wrench 28964778 115.08 USD Add to cart
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Accessory kit for HiScale 50 columns 28966375 150.91 USD Add to cart
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O-ring 48x4 mm 28966655 85.77 USD Add to cart
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Packing tube 20 for HiScale 50 columns 28980251 1 654.61 USD Add to cart
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Make sure you have all connectors and tubing needed for packing and running the column.

Depending on your system configuration and column length, you may need extra long tubing to connect your column.

2x Connector 1/8", 2x Union 5/16" female - 5/16" female, 2x Stop plug 5/16" and 2x Ferrules 1/8" are supplied with the column.

Something went wrong - we were not able to load your agreed MSA pricing. Please try refreshing the page.
# Product Name Product Code Price
1 Tubing 28966376 124.86 USD Add to cart
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2 Connector for 1/8" tubing 18112117 183.85 USD Add to cart
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3 Connector, 5/16" Female - 5/16" Female 18117351 221.00 USD Add to cart
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4 Stop Plug 5/16" Male 18111250 33.21 USD Add to cart
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5 Ferrule for o.d. 1/8" Tubing 18112118 134.04 USD Add to cart
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6 Union 5/16" female - 1/16" male 18114208 52.20 USD Add to cart
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Tubing cutter, for PEEK, EFTE, and FEP tubing i.d. 0.25, 0.5, 0.75, 1 and 1.6 mm 18111246 80.74 USD Add to cart
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Column holder for HiScale 50 28964499 485.31 USD Add to cart
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Accessory kit for HiScale 50 columns 28966375 150.91 USD Add to cart
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Troubleshooting

Find solutions to product related issues. For unlisted issues please contact local Cytiva service representation.

Poor product recovery

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Worrying peak shape

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Drifting baseline

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Poor reproducibility

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Poor packing evaluation result

On this troubleshooting site, we refer to leading and tailing peaks as defined according to the following figures.
 
 

Leading peak Tailing peak

 
Experience shows that the best method of expressing the efficiency of a packed column is in terms of its height equivalent to a theoretical plate (HETP), reduced plate number (h) and its peak asymmetry factor (As). 

Please be aware of that for small columns, less than 10 ml bed volume, the system dead volume has an impact on the column evaluation values.

Issues related to Poor packing evaluation result

General advice to achieve good performance

Before using the system make sure that:

  • The correct system has been selected in UNICORN System Control
  • The correct wavelength has been set for UV/UPC monitor
  • All tubing has been properly connected
  • All connectors are free from leakage
  • No tubing is folded or twisted
  • Online filter, if used, is changed on a regular basis
  • Correct buffers are used for the chosen columns and proteins
  • All inlet tubing has been immersed in correct buffer solutions
  • Enough buffer has been prepared
  • Buffers have been equilibrated to the environment temperature
  • Buffers/eluents have been degassed if necessary (e.g., in RPC runs)
  • Suitable columns have been selected for the target proteins
  • Columns have been cleaned and prepared according to column instructions
  • Samples have been clarified by centrifugation and/or filtration prior to sample loading
  • Samples have been adjusted to binding buffer conditions
  • Auto sampler (if used) has been prepared according to user manual
  • The fraction collector has been filled with appropriate number of microtiter plates or tubes
  • Appropriate arrangement for waste handling has been prepared

Column leakage

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Unsatisfactory elution

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Back pressure increases during operation

Unusual column appearance

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Strange peaks

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