Amersham™ ECL Western Blotting Detection Reagent
Enhanced chemiluminescence (ECL) detection reagents for reliable detection of medium and high expression proteins.
Amersham™ ECL Western Blotting Detection Reagent
Enhanced chemiluminescence (ECL) detection reagents for reliable detection of medium and high expression proteins.
Pack size
Overview
Enhanced chemiluminescence (ECL) detection reagents for reliable detection of medium and high expression proteins.
- Ideal for confirmatory Western blotting applications using chemiluminescence detection
- At least 10-fold more sensitive than colorimetric assays
- Detectable on Amersham Hyperfilm ECL or with a CCD imager such as ImageQuant LAS 500.
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High sensitivity chemiluminescence detection on Western blotting
Amersham ECL Western Blotting Detection Reagents use enhanced luminol-based detection, which is suitable for all routine Western blotting. ECL Western Blotting Detection Reagents, in combination with Amersham Protran membranes, form a complete solution for Western blot applications.
Reliable chemiluminescence detection
In most contemporary ECL systems a luminol peroxide detection reagent is added to the membrane and reacts with the horseradish peroxidase enzyme (HRP) conjugated to the secondary antibody. HRP catalyzes the oxidation of luminol in a multistep reaction and is accompanied by the emission of low-intensity light at 428 nm, which can be measured with light-sensitive X-ray film or with a CCD imager.
