GST bulk kit
GST bulk kit is designed for small-scale manual purification of GST-tagged proteins using either column chromatography or batch method. Facilitates optimization studies of expression, solubility conditions and purification parameters of GST-tagged proteins
GST bulk kit
GST bulk kit is designed for small-scale manual purification of GST-tagged proteins using either column chromatography or batch method. Facilitates optimization studies of expression, solubility conditions and purification parameters of GST-tagged proteins
Overview
- For rapid purification of GST-tagged proteins using gravity flow columns or using batch purification with high specificity.
- Preserves protein antigenicity and function.
- Yields up to 50 mg of pure protein.
GST Bulk Kit contains a 10 ml bulk pack of Glutathione Sepharose 4B medium, five gravity-flow columns, IPTG, and GST Buffer Kit. GST-tagged proteins can be recovered under mild elution conditions (10 mM glutathione), which preserves functionality of the proteins. The binding capacity of Glutathione Sepharose 4B, which can be greater than 10 mg protein/ml medium, varies with the nature of the tagged protein and the exact binding conditions used.
GST Bulk Kit is designed for small-scale manual purification of GST-tagged proteins using either column chromatography or a batch method. The batch method is more flexible, as purification can be performed with between 10 μl and 2 ml of Glutathione Sepharose 4B.
GST Bulk Kit also facilitates optimization studies of expression, solubility conditions, and purification parameters of GST-tagged proteins. The kit contains medium and reagents for purification of GST-tagged proteins from milliliter volumes of sample to larger volumes of sample containing as much as 50 mg of GST-tagged protein.
