HyClone™ Cell Boost™ 4 Supplement
Fed-batch media supplement containing amino acids, vitamins, glucose, trace elements, and growth factors for CHO cells. Liquid and powder.
HyClone™ Cell Boost™ 4 Supplement
Fed-batch media supplement containing amino acids, vitamins, glucose, trace elements, and growth factors for CHO cells. Liquid and powder.
Pack size
Overview
Supplements cell culture with amino acids, vitamins, glucose, trace elements, and growth factors. Manufactured to meet cGMP manufacturing standards and QC specifications.
- Designed to support modern manufacturing processes, Cell Boost™ 4 is chemically defined (CD), animal-derived component-free (ADCF), protein-free, and hydrolysate-free.
- For convenience, this cell culture supplement is available as a ready-to-use liquid feed solution in addition to a dry powder format.
Improve productivity and cell yield in fed-batch cultures by supplementing the cell culture medium to replace nutrients depleted during cell culture. HyClone™ Cell Boost™ 4 Supplement provides amino acids, vitamins, glucose, trace elements, and growth factors to support fed-batch cultures suitable for research and bioprocessing applications. It is available as a convenient ready-to-use liquid feed solution or as a dry powder feed to be hydrated before use.
Fed-batch applications
This HyClone™ cell culture supplement has been developed for recombinant protein production with various cell lines including Chinese hamster ovary (CHO) cell line.
General culture recommendations
Supplement 100 to 200 mL/L, one or more times, starting during early- to mid-growth phase. This is expected to be day 2 to 4. Start the culture at a reduced volume according to anticipated feed volumes. Continue supplementation daily until the final volume is reached. It is recommended to monitor L- glutamine separately and supplement as needed.
Dry powdered medium milling equivalency study
This study compares pin milling of powdered HyClone™ cell culture media and supplements at two manufacturing sites. Detailed physical, chemical, and biological data, including cell growth, are provided.
