Exonuclease I
Exonuclease I catalyzes sequential removal of nucleotides from the 3' end of single-stranded DNA molecules.
Exonuclease I
Exonuclease I catalyzes sequential removal of nucleotides from the 3' end of single-stranded DNA molecules.
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Overview
Exonuclease I is used to selectively digest excess single stranded DNA in a mixture also containing double-stranded DNA, such as removing unused primers after PCR.
- 3'→5' single-stranded exonuclease.
- Monomeric 54.5 kDa bacterial exonuclease I recombinantly expressed in E. coli.
- Inactivated by heating at 80°C for 15 minutes.
- Active in PCR buffers.
Exonuclease I, used together with Alkaline Phosphatase for inactivation of dNTPs, provides effective cleanup of PCR reactions for subsequent use in DNA sequencing.
Digestion of single stranded DNA by Exonuclease I releases dNTPs leaving 5'-terminal dinucleotides intact. DNA strands with terminal 3'-OH groups blocked by phosphoryl or acetyl groups are not removed.
Product specifications
Showing 2 products
Exonuclease I, 5000 units
E70073X
Selling fast5 available
Exonuclease I, 2500 units
E70073Z
Selling fast4 available
Product availability is not guaranteed and subject to change.
