DNA modifying enzymes

Alkaline phosphatase is suitable for dephosphorylation of 5' and 3'-overhangs, recessed ends, blunt ends, and single nucleotides. Following incubation, alkaline phosphatase can be easily inactivated by heating.

Exonuclease I is used to selectively digest excess single stranded DNA in a mixture also containing double-stranded DNA, such as removing unused primers after PCR.

Ready-To-Go T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the 5′-phosphoryl group and the 3′-hydroxyl group of two double-stranded DNA fragments.

Taq DNA Polymerase is a single subunit enzyme purified from the thermophilic bacterium Thermus aquaticus , the native enzyme expressed in E. coli. It polymerizes DNA from a primer annealed to a DNA template in the presence of dNTP for PCR.

Thermo Sequenase™ DNA polymerase is a novel thermostable DNA polymerase that uses ddNTPs as readily as dNTP substrates at temperatures similar to those used in standard PCR. The result is: