Amersham Shrimp Alkaline Phosphatase
Alkaline Phosphatase catalyzes the removal of 5'- and 3'-Phosphate groups from DNA, RNA, Nucleotides and Proteins.
Amersham Shrimp Alkaline Phosphatase
Alkaline Phosphatase catalyzes the removal of 5'- and 3'-Phosphate groups from DNA, RNA, Nucleotides and Proteins.
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Overview
Alkaline phosphatase is suitable for dephosphorylation of 5' and 3'-overhangs, recessed ends, blunt ends, and single nucleotides. Following incubation, alkaline phosphatase can be easily inactivated by heating.
- Bacterial alkaline phosphatase recombinantly expressed in E. coli.
- Active in PCR and restriction endonuclease buffers.
- Inactivated by heating at 75°C for 5 minutes.
Use together with Exonuclease I for cleaning up PCR reactions prior to downstream application such as DNA sequencing.
Unit Definition
One unit is the amount of the enzyme required to dephosphorylate 1 µg of linearized pUC57 DNA termini in 10 minutes at 37°C.
Product specifications
Showing 3 products
Amersham Shrimp Alkaline Phosphatase, 500
E70092Y
Selling fast3 available
Amersham Shrimp Alkaline Phosphatase, 5000
E70092X
Stock on request
Amersham Shrimp Alkaline Phosphatase, 1000
E70092Z
Stock on request
Product availability is not guaranteed and subject to change.
