Superdex™ 30 Increase columns

Superdex 30 Increase SEC columns are for high resolution, small-scale purification and analysis of small biomolecules with M_r ~ 100 to ~ 7000, such as peptides.

Superdex™ 30 Increase columns

Superdex 30 Increase SEC columns are for high resolution, small-scale purification and analysis of small biomolecules with M_r ~ 100 to ~ 7000, such as peptides.

Bed Dimensions

3.2 × 300 mm

10 × 300 mm

Superdex 30 Increase 10/300 GL

29219757

2,864.00 USD

In stock–7 available

Min qty: 1

Price includes standard shipping and handling

Overview

Superdex 30 Increase SEC columns are for high resolution, small-scale purification and analysis of small biomolecules with M_r ~ 100 to ~ 7000, such as peptides.

Improved performance – improved resolution and runtime compared to predecessor Superdex Peptide
Tolerance to repeated harsh cleaning procedures at high pH ensures long column lifetime and minimizes carryover.
Very high resolution – small bead size and narrow particle size distribution provide high resolution, for high protein purity.
High flow rate – due to rigid beads giving excellent pressure/flow properties.

New generation of size exclusion chromatography columns

Superdex 30 Increase is part of Cytiva's new generation of agarose-based SEC columns called “Increase”, which offer higher resolution purification in shorter runtimes compared with their predecessors.

SEC columns for analysis and purification of peptides

Superdex 30 Increase is suitable for both preparative and analytical purposes.

The fractionation range allows preparative separation of peptides, oligosaccharides and other small biomolecules (M_r ~ 100 to ~ 7000) with sample volumes up to 500 µL.

These columns also enable protein characterization, including aggregate analysis, size homogeneity analysis, and interaction studies, where high-quality data can be achieved

Cleaning-in-place (CIP) capabilities

This agarose-based resin is alkali resistant and supports cleaning-in-place (CIP) procedures. This capability allows the same column to be used for different biomolecules, with minimal risk of carry-over between samples.

Regular cleaning can also increase column lifetime.

Two SEC column sizes

Two size exclusion chromatography (SEC) column sizes are designed to serve different purposes:

10/300 GL column, 10 mm × 300 mm, for high resolution analysis as well as for small-scale (25–500 µL) preparative purification, such as the polishing step in a purification protocol
3.2/300 column, 3.2 mm × 300 mm, for high resolution analysis and purification of sample volumes of 4–50 µl, when sample amount is limited and low buffer consumption is important
It is also possible to order Superdex 30 Increase resin to be packed in 5/150 GL column, 5 mm x 150 mm, as custom designed product.