Variant
MabSelect™ VL protein L resin in PreDictor™ 96-well filter plates with a redesigned protein L ligand. For capture of antibody Fabs, bispecifics, and other variants containing a kappa light chain with high binding capacity and improved alkaline stability.
As the diversity of antibody variants increases, so does the need for chromatography resins that can capture and purify these variants. As an alternative to protein A, protein L resin can be used to capture antibody Fabs, bispecific antibodies, and other molecules containing kappa variants of the variable light (VL) chain. Protein L binds human kappa light chain subtypes 1, 3, and 4 and mouse kappa light chain subtype 1.
MabSelect™ VL protein L resin showed a two-fold increase in binding capacity compared to our Capto™ L chromatography resin. This higher capacity provides productivity advantages in research and biomanufacturing.
The MabSelect™ VL protein L resin can withstand cleaning with 0.1 M NaOH, thanks to multimerization and modification of one of the domains of the native protein L ligand.
MabSelect™ VL protein L resin is also available in 1 mL and 5 mL HiTrap™ prepacked columns for use in research applications, HiScreen™ columns and PreDictor™ RoboColumn® minicolumns for convenient method optimization and parameter screening in process development as well as in bulk resins and ReadyToProcess™ columns in different sizes. Contact your Cytiva sales representative for more information.
Learn how to purify bispecific antibodies using MabSelect™ VL protein L resin and how MabSelect™ VL protein L resin offer increased binding capacities versus other protein L resins.