Biacore™ Simul8, you can optimize your assay parameters and verify the affinity and kinetics constants you calculated using a label-free optical biosensor, such as Biacore™ SPR system. Biacore™ Simul8 simulates sensorgrams based on your standard optical biosensor assay parameters, assay format (multi-cycle versus single-cycle), and the selected binding model. The video provides a short demonstration on how you can apply this tool to ensure your affinity and kinetics data are theoretically possible and safe to share or publish.