HisTrap™ FF columns for high-capacity protein purification
Prepacked Ni2+ Sepharose columns for histidine-tagged protein purification
HisTrap™ FF columns for high-capacity protein purification
Prepacked Ni2+ Sepharose columns for histidine-tagged protein purification
Pack size
Overview
Prepacked Ni2+ Sepharose columns for histidine-tagged protein purification
- High binding capacity, approx. 40 mg/mL resin.
- Negligible leakage of Ni2+.
- Prepacked columns offer reliable and convenient time-saving purification of histidinetagged recombinant proteins.
- Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
Ni Sepharose 6 Fast Flow consists of 90 µm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni2+ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni2+ ions from the matrix ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications
The high flow rates made possible by the Ni Sepharose 6 Fast Flow matrix make HisTrap FF columns well suited for small scale-up. Capacity can be increased by connecting columns in series. HiTrap IMAC FF is the product of choice when charging the resin with different metal ions for optimization of purification protocols.
Product specifications
