ReadyToProcess™ Pump 25
The ReadyToProcess Pump 25 has a flow range covering multiple applications, from additions of feed and base to perfusion culturing.
ReadyToProcess™ Pump 25
The ReadyToProcess Pump 25 has a flow range covering multiple applications, from additions of feed and base to perfusion culturing.
Overview
ReadyToProcess Pump 25 is a peristaltic unit incorporating two roller pumps for feed, harvest, and pH control. The pump design makes tubing installation convenient and easy. Tubing sizes from 0.5 mm (1/50”) to 4.8 mm (3/16”) internal diameter can be used to support flow rates from 0.07 to 100 mL/min. Flow rate is regulated by weight feedback from the scale and is controlled by automatic adjustments of the pump speed. Manual adjustment of the flow rate is also possible.
Up to three pump units with all together six pump heads can be connected to the rocker. Up to six pump roles can be selected from a set of 12 different possible options (left: acid, base, feed1, feed2, feed3, or harvest; and right: acid, base, feed1, feed2, feed3, or harvest) for the dual mode and seven different possible options (acid, base, feed1, feed2, feed3, feed4, or harvest) for the single mode.
Medium handling and perfusion using calibrated pumps
Calibration of pumps can be performed manually or automatically during an ongoing perfusion process. Automatic calibration is possible for flow rates above 3.5 L/d and is easily managed by entering information on tubing diameter in the UNICORN software.
Automated pH control
To minimize fluctuations in pH during adjustments with CO2/base or acid/base, the flow rate of the acid/base pumps needs to be rigorously monitored. The ReadyToProcess WAVE 25 system uses combined information from the load cells, the current process pH value, the pump tubing diameter, and the acid/base molarity for calculation of the needed flow rate during the run.
