The larger FineLINE 70[KM1] [LV2] , 100P, 200P, and 350P columns are intended for scale-up work and small-scale production. Column tubes are manufactured in electropolished stainless steel and are available in two tube lengths: 350 mm and 700 mm.

Isolating the column after packing

Use 2- or 4-way stainless steel valves with internal diameter 6 mm to close off the top and bottom of the column and prevent contamination of the bed. For storage purposes, the 25 mm blind flanges with clamps and gaskets can be used to seal off the column.

For FineLINE Pilot 35, an extra SRV-1 valve is inserted to close off the bottom of the column. The stop plug for the upper column inlet is supplied with the column.

Connecting the column to your system

Clamps and gaskets with internal diameter 6 mm are required to connect the 25 mm sanitary flanged inlet/outlet to either valves or tubing of the same type. Preflanged tubing with internal diameter 6 mm is available from Cytiva.

FineLINE Pilot 35 is delivered with flanged 1.2 mm internal diameter propylene tubing and M6 connectors. A separate tubing kit is needed to connect the column to ÄKTA design systems.

Assembly/disassembly of column

Standard wrenches are recommended in a nonexplosive environment. In potentially explosive atmospheres, only tools and protective equipment specially adapted to that environment should be used for operation and maintenance.

Standard wrenches are not supplied with the column, except for FineLINE Pilot 35.

What else do you need?

• The stand for FineLINE 70/70L and FineLine 100P/100PL has adjustable feet. Wheels with brakes are available as an accessory. • The stand for FineLINE 200P/200PL has wheels with brakes as standard. • For FineLINE 70, 100, and 200 columns, the stand must be ordered as an optional component.

Useful spare parts and accessories

• Bed supports: Columns are delivered with 2 µm nets; 10 µm nets are also available. • Seals: Order solvent-resistant seals (O-ring kit PFR) if the EPDM O-rings supplied with the columns are not compatible with the solvent to be used. • A complete set of O-rings, flanging start-up, extra tubing, and connectors. • Pressure gauge: A pressure gauge capable of measuring a pressure of –1 bar at the top mobile phase connection to indicate the pressure in the column is recommended. This monitors the operating pressure and ensures that the correct axial compression packing pressure is set when packing the column. • Pressure relief valve: Required for the packing procedure. It is connected between the pump and the hydraulic inlet to ensure flow delivery at constant pressure. A suitable pressure relief valve designated RL4 is available. The valve is not supplied with the column and should be ordered separately. As the pressure relief valve is only required when packing the column, only one valve will generally be needed irrespective of the number of columns in use. A manometer is seldom needed for FineLINE Pilot 35 since the pressure-relief valve is preset to 10 bar.